Saré Rachel Michelle, Lemons Abigail, Smith Carolyn Beebe
Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20814, USA.
Brain Sci. 2021 Apr 20;11(4):522. doi: 10.3390/brainsci11040522.
Rodent models of brain disorders including neurodevelopmental, neuropsychiatric, and neurodegenerative diseases are essential for increasing our understanding of underlying pathology and for preclinical testing of potential treatments. Some of the most important outcome measures in such studies are behavioral. Unfortunately, reports from different labs are often conflicting, and preclinical studies in rodent models are not often corroborated in human trials. There are many well-established tests for assessing various behavioral readouts, but subtle aspects can influence measurements. Features such as housing conditions, conditions of testing, and the sex and strain of the animals can all have effects on tests of behavior. In the conduct of behavior testing, it is important to keep these features in mind to ensure the reliability and reproducibility of results. In this review, we highlight factors that we and others have encountered that can influence behavioral measures. Our goal is to increase awareness of factors that can affect behavior in rodents and to emphasize the need for detailed reporting of methods.
包括神经发育、神经精神和神经退行性疾病在内的脑部疾病啮齿动物模型，对于增进我们对潜在病理的理解以及对潜在治疗方法进行临床前测试至关重要。此类研究中一些最重要的结果指标是行为学指标。不幸的是，不同实验室的报告往往相互矛盾，并且啮齿动物模型的临床前研究在人体试验中常常得不到证实。有许多成熟的测试可用于评估各种行为读数，但一些细微方面可能会影响测量结果。诸如饲养条件、测试条件以及动物的性别和品系等特征，都可能对行为测试产生影响。在进行行为测试时，牢记这些特征以确保结果的可靠性和可重复性非常重要。在本综述中，我们强调了我们和其他人遇到的可能影响行为测量的因素。我们的目标是提高对可能影响啮齿动物行为的因素的认识，并强调详细报告方法的必要性。
