Alam Aftab, Rehman Najeeb Ur, Ansari Mohd Nazam, Palla Amber Hanif
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Molecules. 2021 Apr 27;26(9):2546. doi: 10.3390/molecules26092546.
The present study examined the chemical composition and antimicrobial and gastrointestinal activity of the essential oils of (L.) Maton harvested in India (EC-I) and Guatemala (EC-G). Monoterpenes were present in higher concentration in EC-I (83.24%) than in EC-G (73.03%), whereas sesquiterpenes were present in a higher concentration in EC-G (18.35%) than in EC-I (9.27%). Minimum inhibitory concentrations (MICs) of 0.5 and 0.25 mg/mL were demonstrated against in EC-G and EC-I, respectively, whereas MICs of 1 and 0.5 mg/mL were demonstrated against in EC-G and EC-I, respectively. The treatment with control had the highest kill-time potential, whereas the treatment with oils had shorter kill-time. EC-I was observed to be more potent in the castor oil-induced diarrhea model than EC-G. At 100 and 200 mg/kg, P.O., EC-I exhibited 40% and 80% protection, respectively, and EC-G exhibited 20% and 60% protection, respectively, in mice, whereas loperamide (10 mg/kg, i.p., positive control) exhibited 100% protection. In the in vitro experiments, EC-I inhibited both carbachol (CCh, 1 µM) and high K (80 mM)-induced contractions at significantly lower concentrations than EC-G. Thus, EC-I significantly inhibited and and exhibited more potent antidiarrheal and antispasmodic effects than EC-G.
本研究检测了在印度（EC-I）和危地马拉（EC-G）采集的（L.）Maton精油的化学成分、抗菌活性和胃肠道活性。单萜类化合物在EC-I中的浓度（83.24%）高于EC-G（73.03%），而倍半萜类化合物在EC-G中的浓度（18.35%）高于EC-I（9.27%）。EC-G和EC-I对的最低抑菌浓度（MIC）分别为0.5和0.25mg/mL，而EC-G和EC-I对的MIC分别为1和0.5mg/mL。对照处理的杀菌时间最长，而精油处理的杀菌时间较短。在蓖麻油诱导的腹泻模型中，观察到EC-I比EC-G更有效。在小鼠中，口服100和200mg/kg的EC-I分别表现出40%和80%的保护作用，EC-G分别表现出20%和60%的保护作用，而洛哌丁胺（10mg/kg，腹腔注射，阳性对照）表现出100%的保护作用。在体外实验中，EC-I在比EC-G显著更低的浓度下抑制了卡巴胆碱（CCh，1µM）和高钾（80mM）诱导的收缩。因此，EC-I显著抑制了和，并且比EC-G表现出更强的止泻和解痉作用。
