Lee Hak-Dong, Kim Ji Hyun, Pang Qi Qi, Jung Pil-Mun, Cho Eun Ju, Lee Sanghyun
Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Department Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea.
Antioxidants (Basel). 2020 Nov 19;9(11):1148. doi: 10.3390/antiox9111148.
This study determined acteoside and its content in via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and O radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, OH, and O radical scavenging activities of leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in leaves, which resulted in DPPH, OH, and O radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.
本研究通过高效液相色谱/紫外检测法（HPLC/UV）和液相色谱/电喷雾电离质谱法（LC/ESI-MS）测定了[植物名称未给出]中的洋丁香酚苷及其含量，以深入了解该植物作为抗氧化剂的潜在用途。此外，还进行了1,1-二苯基-2-苦基肼（DPPH）、羟基（OH）和超氧阴离子（O）自由基清除活性测定，以评估其体外抗氧化活性。[植物名称未给出]叶乙醇提取物在浓度为250μg/mL时的DPPH、OH和超氧阴离子自由基清除活性分别为88.32%、94.48%和14.36%，而相同浓度下茎提取物的清除活性分别为88.15%、88.99%和15.36%。[植物名称未给出]叶和茎中洋丁香酚苷的含量分别为162.11和29.68mg/g。洋丁香酚苷被确定为[植物名称未给出]叶中的主要抗氧化化合物，在浓度为25μg/mL时，其DPPH、OH和超氧阴离子自由基清除活性分别为82.84%、89.46%和30.31%。这些结果表明，含有抗氧化剂洋丁香酚苷的[植物名称未给出]叶和茎可作为功能性和营养补充剂的天然成分。
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