Antioxidant Activity and Acteoside Analysis of .

This study determined acteoside and its content in via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and O radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, OH, and O radical scavenging activities of leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in leaves, which resulted in DPPH, OH, and O radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.