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负氧离子减轻颗粒物诱导的人角质形成细胞系HaCaT中的炎症和氧化应激。

Negative Air Ions Alleviate Particulate Matter-Induced Inflammation and Oxidative Stress in the Human Keratinocyte Cell Line HaCaT.

作者信息

Kim Minjeong, Jeong Guk Jin, Hong Ji Yeon, Park Kui Young, Lee Mi-Kyung, Seo Seong Jun

机构信息

Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Korea.

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea.

出版信息

Ann Dermatol. 2021 Apr;33(2):116-121. doi: 10.5021/ad.2021.33.2.116. Epub 2021 Mar 8.

DOI:10.5021/ad.2021.33.2.116
PMID:33935452
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8082002/
Abstract

BACKGROUND

Recent studies have revealed that particulate matter induces inflammation, oxidative stress, and several skin diseases. Experimental results have also shown that negative air ions are highly effective in removing particulate matter-induced inflammation.

OBJECTIVE

The present study aimed to investigate whether negative air ions can inhibit inflammatory responses and reduce oxidative stress in HaCaT cells exposed to particulate matters.

METHODS

HaCaT cells were treated with particulate matter in the presence or absence of negative air ions and the viability was evaluated by the MTT assay. Reactive oxygen species (ROS) generation was quantified by the dichlorodihydrofluorescein diacetate assay. The expression of genes and proteins was analyzed by real-time polymerase chain reaction and Western blot. Levels of inflammatory cytokines were quantified by enzyme-linked immunosorbent assay.

RESULTS

Negative air ions were observed to downregulate the mRNA and protein levels of particulate matter-induced pro-inflammatory cytokines in HaCaT cells. In addition, negative air ion treatment suppressed particulate matter-induced intracellular ROS generation, p38 mitogen-activated protein kinase activation, and activator protein 1 (c-Fos and c-Jun) activation.

CONCLUSION

Our findings indicate that negative air ions exert anti-inflammatory and antioxidant effects in HaCaT cells exposed to particulate matter. Therefore, negative air ions can be used for the prevention and treatment of particulate matter-related inflammatory skin diseases.

摘要

背景

最近的研究表明，颗粒物可引发炎症、氧化应激及多种皮肤疾病。实验结果还显示，负离子在消除颗粒物引发的炎症方面具有高效性。

目的

本研究旨在探究负离子是否能够抑制暴露于颗粒物环境中的HaCaT细胞的炎症反应并减轻氧化应激。

方法

在有或无负离子存在的情况下，用颗粒物处理HaCaT细胞，通过MTT法评估细胞活力。采用二氯二氢荧光素二乙酸酯法对活性氧（ROS）的生成进行定量分析。通过实时聚合酶链反应和蛋白质印迹法分析基因和蛋白质的表达。采用酶联免疫吸附测定法定量分析炎症细胞因子的水平。

结果

观察到负离子可下调HaCaT细胞中颗粒物诱导的促炎细胞因子的mRNA和蛋白质水平。此外，负离子处理可抑制颗粒物诱导的细胞内ROS生成、p38丝裂原活化蛋白激酶激活以及活化蛋白1（c-Fos和c-Jun）激活。

结论

我们的研究结果表明，负离子对暴露于颗粒物环境中的HaCaT细胞具有抗炎和抗氧化作用。因此，负离子可用于预防和治疗与颗粒物相关的炎症性皮肤病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/edfd6c094ade/ad-33-116-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/c48394afd9a4/ad-33-116-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/6190bf1b13e8/ad-33-116-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/edfd6c094ade/ad-33-116-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/c48394afd9a4/ad-33-116-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/6190bf1b13e8/ad-33-116-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e4f/8082002/edfd6c094ade/ad-33-116-g003.jpg

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