Spedalieri Cecilia, Szekeres Gergo Péter, Werner Stephan, Guttmann Peter, Kneipp Janina
Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
School of Analytical Sciences Adlershof, Humboldt-Universität zu Berlin, Albert-Einstein-Str. 5-9, 12489 Berlin, Germany.
Nanomaterials (Basel). 2021 Apr 30;11(5):1183. doi: 10.3390/nano11051183.
Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics.
金纳米星是一种多功能的等离子体纳米材料，在生物分析中有许多应用。本文在早期内溶酶体处理阶段，从分子和超微结构水平研究了它们与三种不同细胞系动物细胞的相互作用。以金纳米星本身作为表面增强拉曼散射的底物，对其蛋白质冠层和内溶酶体环境中的分子进行了表征。通过低温软X射线纳米断层扫描探测细胞内溶酶体区室中金纳米星聚集体的定位、形态和大小。J774细胞系巨噬细胞对金纳米星的处理与成纤维细胞系3T3和上皮细胞系HCT - 116有很大不同，并且发现相同细胞中生物分子冠层的结构和组成与球形金纳米颗粒相似。用不同形态的金纳米星获得的数据表明，体内表面的生物分子相互作用受尖峰长度影响，尖峰长度越长，与蛋白质和脂质疏水基团的相互作用增加，且与细胞系无关。这些结果将为传感、成像和治疗诊断的优化纳米星合成与递送提供支持。
