Alharbi Bandar, Qanash Husam, Almashjary Majed N, Barnawi Heba, Aldarhami Abdu, Alsaif Ghaida, Alsamaan Fahad, Monjed Mohammad Khalil, Al Shmrany Humood, Bazaid Abdulrahman S
Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha'il, Hail, Saudi Arabia.
Medical and Diagnostic Research Center, University of Ha'il, Hail, Saudi Arabia.
Front Pharmacol. 2024 Aug 23;15:1424369. doi: 10.3389/fphar.2024.1424369. eCollection 2024.
Plant-derived compounds are renowned for their remarkable pharmacological properties, holding immense promise for therapeutic interventions in human health. In this study, we aimed to assess the antimicrobial, anti-hemolytic, antioxidant, and wound healing attributes of watercress oil incorporated into Vaseline gel (OLG) compared to watercress oil alone. OLG was formulated through a meticulous process involving the addition of Vaseline gel to the oil under agitation conditions. High-performance liquid chromatography analysis of watercress oil unveiled a rich array of phenolic compounds, including gallic acid (10.18 μg/mL), daidzein (3.46 μg/mL), and hesperetin (3.28 μg/mL). The inhibitory zones caused by watercress oil alone against a spectrum of pathogens, including and , were measured at 25 ± 0.3, 26 ± 0.1, 22 ± 0.2, 25 ± 0.2, and 24 ± 0.1 mm, respectively. Notably, OLG exhibited slightly larger zones of inhibition (27 ± 0.2, 30 ± 0.2, 24 ± 0.1, 28 ± 0.1, and 25 ± 0.3 mm) against the same microbial strains. Furthermore, the minimum inhibitory concentration (MIC) of OLG against and was lower compared to watercress oil alone, indicating enhanced efficacy. Similarly, the minimum bactericidal concentration (MBC) of OLG was notably lower across all tested bacteria compared to watercress oil alone. Inhibition of bacterial hemolysis, particularly , was significantly enhanced with OLG treatment, showcasing reductions of 19.4%, 11.6%, and 6.8% at 25%, 50%, and 75% MIC concentrations, respectively, compared to watercress oil alone. The antioxidant activity of both oil and OLG was quantified with IC50 values of 2.56 and 3.02 μg/mL, respectively. Moreover, OLG demonstrated remarkable efficacy in wound healing assays, with notable enhancements in migration rate, wound closure, and area difference compared to control cells. In light of the observed antibacterial, antifungal, anti-hemolytic, and wound healing properties of OLG, this formulation holds therapeutic potential in treating microbial infections and promoting wound healing.
植物源化合物以其卓越的药理特性而闻名，在人类健康治疗干预方面具有巨大潜力。在本研究中，我们旨在评估与单独的豆瓣菜油相比，掺入凡士林凝胶中的豆瓣菜油（OLG）的抗菌、抗溶血、抗氧化和伤口愈合特性。OLG是通过在搅拌条件下向油中添加凡士林凝胶的精细过程配制而成的。对豆瓣菜油的高效液相色谱分析揭示了一系列丰富的酚类化合物，包括没食子酸（10.18μg/mL）、大豆苷元（3.46μg/mL）和橙皮素（3.28μg/mL）。单独的豆瓣菜油对包括 和 在内的一系列病原体产生的抑菌圈分别测定为25±0.3、26±0.1、22±0.2、25±0.2和24±0.1mm。值得注意的是，OLG对相同的微生物菌株表现出稍大的抑菌圈（27±0.2、30±0.2、24±0.1、28±0.1和25±0.3mm）。此外，与单独的豆瓣菜油相比，OLG对 和 的最低抑菌浓度（MIC）更低，表明其功效增强。同样，与单独的豆瓣菜油相比，OLG在所有测试细菌中的最低杀菌浓度（MBC）明显更低。用OLG处理可显著增强对细菌溶血的抑制作用（尤其是 ），与单独的豆瓣菜油相比，在25%、50%和75%的MIC浓度下分别降低了19.4%、11.6%和6.8%。油和OLG的抗氧化活性分别用IC50值2.56和3.02μg/mL进行定量。此外，在伤口愈合试验中，OLG表现出显著的功效，与对照细胞相比，迁移率、伤口闭合和面积差异都有显著提高。鉴于观察到的OLG的抗菌、抗真菌、抗溶血和伤口愈合特性，这种制剂在治疗微生物感染和促进伤口愈合方面具有治疗潜力。
