Istituto Zooprofilattico Sperimentale della Sardegna, Struttura Complessa di Microbiologia e Ispezione degli Alimenti di Origine Animale, Via Duca degli Abruzzi 8, 07100 Sassari, Italy.
Agenzia Regionale per la Protezione dell'Ambiente della Sardegna, Via Rockefeller 58/60, 07100 Sassari, Italy.
J Food Prot. 2021 Sep 1;84(9):1549-1554. doi: 10.4315/JFP-21-059.
In the present study, we investigated the presence, seasonal distribution, and biomolecular characteristics of Vibrio parahaemolyticus and Vibrio vulnificus in samples of bivalve mollusks (Mytilus galloprovincialis, Crassostrea gigas, and Ruditapes decussatus) harvested and marketed in Sardinia (Italy) between 2017 and 2018. A total of 435 samples were submitted for qualitative determination of Vibrio spp., V. parahaemolyticus, and V. vulnificus. Potentially enteropathogenic isolates were detected with biomolecular methods. The overall prevalence of Vibrio spp. was 7.6%. The highest Vibrio prevalence was found in R. decussatus (8.3%). The prevalences of V. parahaemolyticus and V. vulnificus were 2.7 and 4.8%, respectively. Higher prevalences of V. parahaemolyticus and V. vulnificus were found in R. decussatus (4.2%) and C. gigas (6.2%), respectively. Only two pathogenic V. parahaemolyticus strains were recovered (genotypes: tdh- and trh+; tdh+ and trh-), both from M. galloprovincialis. None of the isolates were tdh+ and trh+. Pathogenic Vibrio infections are often underestimated, and human infections are increasing in Europe. European data on the true distribution of Vibrionaceae are scarce, and the results of the present study highlight the need of constant monitoring to update the distribution of pathogenic vibrios.
本研究调查了 2017 年至 2018 年间在意大利撒丁岛采集和销售的双壳贝类（贻贝、牡蛎和缢蛏）样品中副溶血性弧菌和创伤弧菌的存在、季节性分布和生物分子特征。共提交了 435 份样品用于定性检测弧菌属、副溶血性弧菌和创伤弧菌。采用生物分子方法检测潜在的肠致病性分离株。弧菌属的总流行率为 7.6%。Ruditapes decussatus（8.3%）中副溶血性弧菌的流行率最高。副溶血性弧菌和创伤弧菌的流行率分别为 2.7%和 4.8%。Ruditapes decussatus（4.2%）和牡蛎（6.2%）中副溶血性弧菌和创伤弧菌的流行率较高。仅从贻贝中回收了两种致病性副溶血性弧菌菌株（基因型：tdh+和 trh+；tdh+和 trh-）。所有分离株均未检出 tdh+和 trh+。致病性副溶血性弧菌感染常常被低估，并且在欧洲，人类感染的数量正在增加。关于欧洲振发性弧菌科的确切分布的数据很少，本研究结果强调需要不断监测以更新致病性弧菌的分布。
