Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Res Notes. 2021 May 6;14(1):169. doi: 10.1186/s13104-021-05586-9.
Uncoupling protein 2 (UCP2) plays a crucial role in energy homeostasis via insulin secretion regulation, free fatty acid concentrations, and lipid metabolism. This study aimed to investigate the association of 45-bp ins/del polymorphism of UCP2 with susceptibility to NAFLD (Non-Alcoholic Fatty Liver Disease) and T2DM (Type 2 Diabetes Mellitus). DNA was extracted from the white blood cells of the subjects, and the gene polymorphism was determined using polymerase chain reaction (PCR). In this study, 72 patients with NAFLD, 71 healthy individuals as control, 80 patients with T2DM, and 77 healthy controls were enrolled in the study.
A higher prevalence of insertion/insertion genotype was observed in T2DM patients compared to the controls (p- value˂ 0.05). There was no difference in genotype distribution between NAFLD patients and controls (p-value > 0.05). NAFLD patients with D/D, D/I genotype had higher triglyceride, ALT, and AST levels; however, their HDL levels were lower than healthy controls. Patients with T2DM with D/D or D/I genotype also had significantly higher fasting serum glucose (FSG). While we found an association between the 45 bp I/D polymorphism in 3'UTR of UCP2 and T2DM, no correlation between this polymorphism and NAFLD was identified.
解偶联蛋白 2（UCP2）通过调节胰岛素分泌、游离脂肪酸浓度和脂代谢在能量平衡中发挥关键作用。本研究旨在探讨 UCP2 45-bpins/del 多态性与非酒精性脂肪性肝病（NAFLD）和 2 型糖尿病（T2DM）易感性的关系。从受试者的白细胞中提取 DNA，采用聚合酶链反应（PCR）确定基因多态性。本研究共纳入 72 例 NAFLD 患者、71 例健康对照者、80 例 T2DM 患者和 77 例健康对照者。
T2DM 患者的插入/插入基因型的患病率高于对照组（p-值<0.05）。NAFLD 患者和对照组之间的基因型分布无差异（p 值>0.05）。D/D、D/I 基因型的 NAFLD 患者的甘油三酯、ALT 和 AST 水平较高，而 HDL 水平较低。D/D 或 D/I 基因型的 T2DM 患者的空腹血清葡萄糖（FSG）也显著升高。虽然我们发现 UCP2 3'UTR 中的 45bp I/D 多态性与 T2DM 之间存在关联，但该多态性与 NAFLD 之间没有相关性。
