Saxne T, Heinegård D, Wollheim F A
Department of Rheumatology, University Hospital, Lund, Sweden.
Arthritis Rheum. 1987 Sep;30(9):972-9. doi: 10.1002/art.1780300903.
Proteoglycan concentrations in knee joint synovial fluid and in serum from patients with various inflammatory arthritides were studied using an enzyme-linked immunosorbent assay. Patients with reactive arthritis, calcium pyrophosphate arthropathy, and juvenile rheumatoid arthritis (age less than or equal to 20 years) had the highest synovial fluid concentrations. These values differed significantly (P less than 0.001) from those in patients with rheumatoid arthritis, psoriatic arthropathy, and chronic HLA-B27-associated arthropathy. Rheumatoid arthritis patients receiving low-dose prednisolone treatment had higher synovial fluid (P = 0.006) and serum (P less than 0.001) proteoglycan concentrations than did those taking nonsteroidal antiinflammatory drugs or slow-acting antirheumatic drugs. Serum proteoglycan concentrations were near the detection limits, and did not correlate with levels found in paired samples of knee joint synovial fluid. Patients with calcium pyrophosphate arthropathy had the highest mean serum level of proteoglycan. This assay of proteoglycan antigens is a useful tool in the study of proteoglycan metabolism in patients with joint disease. With its use, differences between disease groups and effects of therapy can be distinguished.
采用酶联免疫吸附测定法研究了各种炎性关节炎患者膝关节滑液和血清中的蛋白聚糖浓度。反应性关节炎、焦磷酸钙关节病和幼年类风湿关节炎（年龄小于或等于20岁）患者的滑液浓度最高。这些值与类风湿关节炎、银屑病关节炎和慢性HLA - B27相关关节病患者的值有显著差异（P小于0.001）。接受低剂量泼尼松龙治疗的类风湿关节炎患者的滑液（P = 0.006）和血清（P小于0.001）蛋白聚糖浓度高于服用非甾体抗炎药或慢作用抗风湿药的患者。血清蛋白聚糖浓度接近检测限，与膝关节滑液配对样本中的水平无关。焦磷酸钙关节病患者的蛋白聚糖平均血清水平最高。这种蛋白聚糖抗原检测方法是研究关节疾病患者蛋白聚糖代谢的有用工具。通过使用该方法，可以区分疾病组之间的差异和治疗效果。
