程序性细胞死亡配体 1 表达的综合分析显示高级别神经胶质瘤中水平升高。
Integrated analysis of programmed cell death ligand 1 expression reveals increased levels in high-grade glioma.
机构信息
Institute of Pathology, University Hospital Salzburg, Paracelsus Medical University, Müllner Hauptstr. 48, 5020, Salzburg, Austria.
Department of Neurosurgery, University Hospital Salzburg, Paracelsus Medical University, Ignatz-Harrer-Str. 79, 5020, Salzburg, Austria.
出版信息
J Cancer Res Clin Oncol. 2021 Aug;147(8):2271-2280. doi: 10.1007/s00432-021-03656-w. Epub 2021 May 8.
PURPOSE
Gliomas are the most frequent primary brain tumors of adults. Despite intensive research, there are still no targeted therapies available. Here, we performed an integrated analysis of glioma and programmed cell death ligand 1 (PD-L1) in 90 samples including 58 glioma and 32 control brain tissues.
METHODS
To identify PD-L1 expression in glioma, we performed immunohistochemical analysis of PD-L1 tumor proportion score (TPS) using the clinically valid PD-L1 22C3 antibody on 90 samples including controls and WHO grade I-IV gliomas.
RESULTS
We found that PD-L1 is highly expressed in a subfraction of glioma cells. Analysis of PD-L1 levels in different glioma subtypes revealed a strong intertumoral variation of PD-L1 protein. Furthermore, we correlated PD-L1 expression with molecular glioma hallmarks such as MGMT-promoter methylation, IDH1/2 mutations, TERT promoter mutations and LOH1p/19q.
CONCLUSION
In summary, we found that PD-L1 is highly expressed in a subfraction of glioma, indicating PD-L1 as a potential new marker in glioma assessment opening up novel therapeutic approaches.
目的
神经胶质瘤是成人最常见的原发性脑肿瘤。尽管进行了深入的研究，但目前仍没有可用的靶向治疗方法。在这里，我们对 90 个样本（包括 58 个神经胶质瘤和 32 个对照脑组织）进行了神经胶质瘤和程序性细胞死亡配体 1（PD-L1）的综合分析。
方法
为了鉴定神经胶质瘤中的 PD-L1 表达，我们使用临床上有效的 PD-L1 22C3 抗体对 90 个样本（包括对照和 WHO 分级 I-IV 神经胶质瘤）进行了 PD-L1 肿瘤比例评分（TPS）的免疫组织化学分析。
结果
我们发现 PD-L1 在神经胶质瘤细胞的一个亚群中高度表达。对不同神经胶质瘤亚型中 PD-L1 水平的分析显示 PD-L1 蛋白存在强烈的肿瘤间变异性。此外，我们还将 PD-L1 表达与分子神经胶质瘤标志物（如 MGMT 启动子甲基化、IDH1/2 突变、TERT 启动子突变和 LOH1p/19q）相关联。
结论
总之，我们发现 PD-L1 在神经胶质瘤的一个亚群中高度表达，表明 PD-L1 作为神经胶质瘤评估的一个潜在新标志物，为新的治疗方法开辟了道路。
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