State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Biliary tract disease institute, Fudan University, Shanghai 200032, China.
Acta Biomater. 2021 Jul 1;128:514-522. doi: 10.1016/j.actbio.2021.04.051. Epub 2021 May 5.
Gallbladder cancer can be difficult to detect in its early stages and is prone to metastasize, causing bile duct obstruction, which is usually treated by stent implantation in clinic. However, the commonly used biliary stents are non-degradable, which not only prone to secondary blockage, but also need to be removed by secondary surgery. Biodegradable magnesium (Mg) is expected to one of the promising candidates for degradable biliary stents due to its excellent physicochemical property and biocompatibility. In this work, we studied the influence of high-purity Mg wires on gallbladder cancer through in vitro and in vivo experiments and revealed that the degradation products of Mg could significantly inhibit the growth of gallbladder cancer cells and promote their apoptosis. Our findings indicate that Mg biliary stent possesses the function of draining bile and treating gallbladder cancer, suggesting that Mg has good application prospects in biliary surgery. STATEMENT OF SIGNIFICANCE: Current research and development of biomedical magnesium are mainly concentrated in the cardiovascular and orthopedics field. Degradable magnesium bile duct stents have great application prospects in the treatment of bile duct blockage caused by bile duct-related cancers. At present, the effect of magnesium implants on gallbladder cancer is not clear. Our work verified the effectiveness of magnesium wire implants in inhibiting gallbladder cancer through in vivo and in vitro experiments, and studied the effect of magnesium degradation products on gallbladder cancer cells from the perspective of cell proliferation, apoptosis and cycle. This study provided new understanding for the application of magnesium in biliary surgery.
胆囊癌在早期阶段难以检测，且易于转移，导致胆管阻塞，临床上通常采用支架植入治疗。然而，常用的胆道支架不可降解，不仅容易再次堵塞，而且需要再次手术取出。可降解镁（Mg）因其优异的物理化学性质和生物相容性，有望成为可降解胆道支架的候选材料之一。在这项工作中，我们通过体外和体内实验研究了高纯 Mg 丝对胆囊癌的影响，结果表明 Mg 的降解产物可显著抑制胆囊癌细胞的生长并促进其凋亡。我们的研究结果表明，Mg 胆道支架具有引流胆汁和治疗胆囊癌的功能，提示 Mg 在胆道手术中有良好的应用前景。
目前生物医用镁的研究与开发主要集中在心血管和骨科领域。可降解镁胆管支架在治疗胆管相关癌症引起的胆管阻塞方面具有广阔的应用前景。目前，镁植入物对胆囊癌的影响尚不清楚。我们的工作通过体内和体外实验验证了镁丝植入物抑制胆囊癌的有效性，并从细胞增殖、凋亡和周期的角度研究了镁降解产物对胆囊癌细胞的影响。这项研究为镁在胆道手术中的应用提供了新的认识。
