Zhu Xiaolin, Wang Baoqiang, Wang Xian, Zhang Chaoyang, Wei Xiaohong
College of Agronomy, Gansu Agricultural University, Lanzhou, 730070 China.
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070 China.
Physiol Mol Biol Plants. 2021 Apr;27(4):787-800. doi: 10.1007/s12298-021-00988-2. Epub 2021 Apr 13.
Lim family members play an important role in the regulation of plant cell development and stress response. However, there are few studies on LIM family in quinoa. In this study, we identified nine LIMS (named cqlim01-cqlim09) from quinoa, which were divided into three subfamilies (α Lim1, γ lim2 and δ lim2) according to phylogeny. The differences in gene structure and motif composition among different subfamilies have been observed. In addition, we studied the repetitive events of the members of the family. The Ka/Ks (non synchronous substitution rate / synchronous substitution rate) ratio analysis showed that the repetitive CqLIMs probably experienced purifying selection pressure. Promoter analysis showed that the family genes contained a variety of hormones, stress and tissue-specific expression elements, and protein interactions showed that these genes had actin stabilizing effect. In addition, QRT PCR results showed that all CqLIM genes were positively regulated under three stresses (low temperature, salt and ABA). These results provide a theoretical basis of further study of LIM gene in quinoa.
The online version contains supplementary material available at 10.1007/s12298-021-00988-2.
林氏家族成员在植物细胞发育和应激反应的调控中发挥着重要作用。然而，关于藜麦中林氏家族的研究较少。在本研究中，我们从藜麦中鉴定出9个LIMs（命名为cqlim01 - cqlim09），根据系统发育将其分为三个亚家族（α Lim1、γ lim2和δ lim2）。观察到不同亚家族之间基因结构和基序组成的差异。此外，我们研究了该家族成员的重复事件。Ka/Ks（非同义替换率/同义替换率）比值分析表明，重复的CqLIMs可能经历了纯化选择压力。启动子分析表明，该家族基因包含多种激素、应激和组织特异性表达元件，蛋白质相互作用表明这些基因具有肌动蛋白稳定作用。此外，QRT PCR结果表明，所有CqLIM基因在三种胁迫（低温、盐和ABA）下均受到正向调控。这些结果为进一步研究藜麦中的LIM基因提供了理论依据。
在线版本包含可在10.1007/s12298 - 021 - 00988 - 2获取的补充材料。
