College of Life Science, Shanxi Datong University, Datong 037009, China.
Research and Development Center of Agricultural Facility Technology, Shanxi Datong University, Datong 037009, China.
Genes (Basel). 2019 Jun 30;10(7):500. doi: 10.3390/genes10070500.
The NAC (NAM, ATAF, and CUC) family is one of the largest families of plant-specific transcription factors. It is involved in many plant growth and development processes, as well as abiotic/biotic stress responses. So far, little is known about the NAC family in . In the present study, a total of 90 were identified in quinoa (named as ) and phylogenetically divided into 14 distinct subfamilies. Different subfamilies showed diversities in gene proportions, exon-intron structures, and motif compositions. In addition, 28 duplication events were investigated, and a strong subfamily preference was found during the expansion in quinoa, indicating that the duplication event was not random across subfamilies during quinoa evolution. Moreover, the analysis of Ka/Ks (non-synonymous substitution rate/synonymous substitution rate) ratios suggested that the duplicated might have mainly experienced purifying selection pressure with limited functional divergence. Additionally, 11 selected showed significant tissue-specific expression patterns, and all the were positively regulated in response to salt stress. The result provided evidence for selecting candidate genes for further characterization in tissue/organ specificity and their functional involvement in quinoa's strong salinity tolerance.
NAC（NAM、ATAF 和 CUC）家族是植物特异性转录因子中最大的家族之一。它参与许多植物生长和发育过程，以及非生物/生物胁迫反应。到目前为止，关于 的 NAC 家族知之甚少。在本研究中，总共鉴定了 90 个藜麦（命名为 ），并在系统发育上分为 14 个不同的亚家族。不同的亚家族在基因比例、外显子-内含子结构和基序组成上表现出多样性。此外，研究了 28 个复制事件，发现藜麦中 扩张时存在强烈的亚家族偏好，表明在藜麦进化过程中，复制事件并不是在 亚家族之间随机发生的。此外，Ka/Ks（非同义替换率/同义替换率）比值的分析表明，复制的 可能主要经历了纯化选择压力，功能分化有限。此外，11 个选定的 表现出明显的组织特异性表达模式，所有的 都对盐胁迫呈正调控。研究结果为进一步鉴定组织/器官特异性的候选基因及其在藜麦耐盐性中的功能参与提供了证据。
