State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, College of Agronomy, Northwest A&F University, Yangling 712100, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China.
Int J Mol Sci. 2019 Mar 15;20(6):1303. doi: 10.3390/ijms20061303.
LIM proteins have been found to play important roles in many life activities, including the regulation of gene expression, construction of the cytoskeleton, signal transduction and metabolic regulation. Because of their important roles in many aspects of plant development, genes have been studied in many plant species. However, the gene family has not yet been characterized in foxtail millet. In this study, we analyzed the whole genome of foxtail millet and identified 10 genes. All gene promoters contain MYB and MYC -acting elements that are related to drought stress. Based on the presence of multiple abiotic stress-related -elements in the promoter of , we chose this gene for further study. We analyzed expression under abiotic stress and hormone treatments using qRT-PCR. We found that was induced by various abiotic stresses and hormones. Under drought conditions, transgenic rice of -overexpression had a higher survival rate, higher relative water content and less cell damage than wild type (WT) rice. These results indicate that overexpression of the foxtail millet gene enhances drought tolerance in transgenic rice, and the gene can potentially be used for molecular breeding of crops with increased resistance to abiotic stress.
LIM 蛋白在许多生命活动中发挥着重要作用，包括基因表达调控、细胞骨架构建、信号转导和代谢调节。由于它们在植物发育的许多方面都具有重要作用，因此在许多植物物种中都对其基因进行了研究。然而，在谷子中，LIM 基因家族尚未得到描述。在这项研究中，我们分析了谷子的全基因组，鉴定了 10 个基因。所有基因的启动子都包含与干旱胁迫相关的 MYB 和 MYC 作用元件。基于 在启动子中存在多种非生物胁迫相关元件，我们选择该基因进行进一步研究。我们使用 qRT-PCR 分析了非生物胁迫和激素处理下 的表达情况。我们发现 受到各种非生物胁迫和激素的诱导。在干旱条件下，过表达谷子 基因的转基因水稻比野生型（WT）水稻具有更高的存活率、更高的相对水含量和更少的细胞损伤。这些结果表明，过表达谷子 基因增强了转基因水稻的耐旱性，该基因可用于培育对非生物胁迫具有更高抗性的作物的分子育种。
