Li Xiaodong, Azhati Baihetiya, Wang Wenguang, Rexiati Mulati, Xing Chen, Wang Yujie
Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
Oncol Lett. 2021 Jun;21(6):486. doi: 10.3892/ol.2021.12747. Epub 2021 Apr 21.
Prostate cancer (PCa) is a common male malignant disease with a high incidence, which can seriously affect the quality of life of patients. The survival rate of patients with PCa has improved to 98.6%; however, new insights for the molecular mechanism are still urgently required. Circular RNA (circ)UBAP2 is a tumor-associated circRNA that has been demonstrated to promote the progression of various types of cancer. CircUBAP2 has been demonstrated to be significantly upregulated in PCa, but its role in the progression of PCa remains unclear. The present study aimed to provide an improved understanding of the regulatory mechanism of circUBAP2 in PCa. circUBAP2 expression was identified to be upregulated in four PCa cell lines and clinical tissues by using reverse transcription-quantitative PCR analysis. Binding sites analysis and luciferase reporter gene assay indicated that the microRNA(miR)-1244/MAP3K2 axis was the target of circUBAP2. Gain-of-function assays revealed that circUBAP2 promoted the proliferation of PCa cells by sponging miR-1244 and promoting the MAP3K2 axis. The present findings may be essential for providing new strategies in the diagnosis and targeted therapy of PCa.
前列腺癌（PCa）是一种常见的男性恶性疾病，发病率高，会严重影响患者的生活质量。PCa患者的生存率已提高到98.6%；然而，对其分子机制仍迫切需要新的见解。环状RNA（circ）UBAP2是一种与肿瘤相关的环状RNA，已被证明可促进各类癌症的进展。circUBAP2在PCa中已被证明显著上调，但其在PCa进展中的作用仍不清楚。本研究旨在更好地理解circUBAP2在PCa中的调控机制。通过逆转录定量PCR分析，发现在四种PCa细胞系和临床组织中circUBAP2表达上调。结合位点分析和荧光素酶报告基因检测表明，微小RNA（miR）-1244/MAP3K2轴是circUBAP2的作用靶点。功能获得实验表明，circUBAP2通过吸附miR-1244并促进MAP3K2轴来促进PCa细胞增殖。本研究结果可能对提供PCa诊断和靶向治疗的新策略至关重要。
