Kumar Ashwini, Bhattarai Ajay, Rathore Aayushi Singh, Jat Gograj Singh, Behera Tusar Kanti, Renukadevi P, Baranwal Virendra K, Jain Rakesh Kumar, Basavaraj Y B
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012 India.
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012 India.
Virusdisease. 2021 Mar;32(1):183-185. doi: 10.1007/s13337-020-00645-4. Epub 2021 Mar 20.
Symptoms like bright yellowing, puckering of the leaf, vein banding, and vein thickening were observed on different cucurbit hosts at the experimental farm of Indian Agricultural Research Institute, New Delhi during 2019. Leaf-dip electron microscopy of the symptomatic leaves revealed the association of isometric virus particles measuring ~ 25 nm with bitter gourd and cucumber samples. The RT-PCR assay using polerovirus generic primers covering the partial RdRp, intergenic region, and partial CP region was resulted the amplicons of ~ 1.1 kb. Subsequent cloning, sequencing, and sequence analysis revealed the association of cucurbit aphid-borne yellows virus (CABYV) with bitter gourd (Momordica charantia) and cucumber () plants. These results constitute the first report of CABYV infection on cucumber plants from India.
The online version of this article (10.1007/s13337-020-00645-4) contains supplementary material, which is available to authorized users.
2019年期间，在新德里印度农业研究所的试验农场中，在不同的葫芦科寄主上观察到叶片亮黄、起皱、脉带和叶脉增厚等症状。对有症状叶片进行叶浸电子显微镜观察，发现约25纳米的等轴病毒颗粒与苦瓜和黄瓜样本有关联。使用覆盖部分RNA依赖的RNA聚合酶（RdRp）、基因间隔区和部分衣壳蛋白（CP）区域的马铃薯卷叶病毒属通用引物进行逆转录聚合酶链反应（RT-PCR）检测，得到了约1.1千碱基对的扩增子。随后的克隆、测序和序列分析表明，葫芦科蚜传黄化病毒（CABYV）与苦瓜（苦瓜属）和黄瓜植株有关联。这些结果构成了印度黄瓜植株感染CABYV的首次报道。
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