Jiang Mei, Li Yuning, Chen Haimei, Wang Bin, Liu Chang
Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.
School of Pharmaceutical Sciences, Xiangnan University, Chenzhou, P. R. China.
Mitochondrial DNA B Resour. 2021 Apr 22;6(4):1498-1506. doi: 10.1080/23802359.2021.1911700.
is a biennial plant of genus belonging to the Asteraceae family. The classification of is controversial, and no consistent conclusions have been reached based on the analysis of morphological characters and different molecular markers. Here, we sequenced and assembled the complete chloroplast genome of . This genome has a total length of 152,339 bp, a conservative quartile structure that is composed of a large single-copy (LSC) region of 83,976 bp, a small-copy (SSC) region of 18,521 bp, and a pair of inverted repeats (IRs) of 24,921 bp. The genome contains 112 unique genes, including 79 protein-coding, four rRNA, and 29 tRNA genes. Repeat analysis obtained 17 microsatellite, 16 tandem, and 17 interspersed repeats. Comparison of sequence divergence between and found the intergenic spacer C-N exhibited the highest degree of variation. Three phylogenetic trees based on the 72 shared protein, K gene, and L gene sequences showed that and are clustered together. The acquisition and comparative analysis of the chloroplast genome provide a valuable resource for the taxonomic and phylogenetic studies of .
是菊科属的一种二年生植物。的分类存在争议,基于形态特征分析和不同分子标记尚未得出一致结论。在此,我们对的叶绿体全基因组进行了测序和组装。该基因组全长152,339 bp,具有保守的四分体结构,由一个83,976 bp的大单拷贝(LSC)区域、一个18,521 bp的小单拷贝(SSC)区域和一对24,921 bp的反向重复序列(IRs)组成。该基因组包含112个独特基因,包括79个蛋白质编码基因、4个rRNA基因和29个tRNA基因。重复序列分析得到17个微卫星、16个串联重复和17个散布重复。和之间的序列分歧比较发现基因间隔区C-N变异程度最高。基于72个共享蛋白质、K基因和L基因序列构建的三个系统发育树表明和聚在一起。叶绿体基因组的获取和比较分析为的分类学和系统发育研究提供了宝贵资源。