Khlebodarova T M, Karasik G I, Matveeva N M, Serov O L, Golovin S Y, Bondar A A, Karginov V A, Morozov I S, Zelenin S M, Mertvetsov N P
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk.
Genomics. 1988 Apr;2(3):185-8. doi: 10.1016/0888-7543(88)90001-8.
A cDNA library from the mink pituitary was screened using as probe a synthetic oligodeoxyribonucleotide, 5'-TTCATGACCTCCGA-3', corresponding to the endorphin region of bovine proopiomelanocortin (POMC) cDNA. As a result, several clones containing inserts complementary to POMC mRNA were identified. The sequence of one of the fragments (585 bp, 65% of the total length of mRNA) was determined. A high degree of homology (over 80%) among the primary structures of sequences from mink, man, and bovine cDNA POMC was established. With the cloned mink cDNA fragment as probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of mink POMC sequences and mink chromosomes in the mink-Chinese hamster panel allowed us to assign the POMC gene to mink chromosome 11.
使用与牛阿黑皮素原（POMC）cDNA的内啡肽区域相对应的合成寡脱氧核糖核苷酸5'-TTCATGACCTCCGA-3'作为探针，筛选水貂垂体的cDNA文库。结果，鉴定出了几个含有与POMC mRNA互补插入片段的克隆。测定了其中一个片段的序列（585 bp，占mRNA全长的65%）。水貂、人和牛cDNA POMC序列的一级结构之间建立了高度同源性（超过80%）。以克隆的水貂cDNA片段为探针，研究了水貂-中国仓鼠杂交克隆的DNA。在水貂-中国仓鼠细胞系中对水貂POMC序列和水貂染色体进行分离分析的结果使我们能够将POMC基因定位到水貂的11号染色体上。
