Department of Pathology, Krishna Institute of Medical Sciences (Deemed to University), MAHARASHTRA, INDIA.
Turk Patoloji Derg. 2021;37(2):145-153. doi: 10.5146/tjpath.2021.01524.
Acute Myeloid Leukemia (AML) and Acute Lymphoblastic Leukemia (ALL) are clinically and biologically diverse phenotypic diseases amongst hematological malignancies. The current study objectives were to diagnose and classify cases of AL as per revised 4th edition of WHO 2016 classification of AL's and study their clinicopathological profiles.
This cross-sectional, observational study included 68 patients, diagnosed with AL were recruited. Diagnosis was based on peripheral blood smear examination, bone marrow aspiration, flowcytometry, and cytogenetic and molecular studies.
Sixty-eight cases of AL were diagnosed in a period of 2 years, where 25 cases were of ALL and 43 cases were of AML. In the subclassification of AML as per WHO 2016, 20 cases were of AML, RGA, 21 cases were of AML, NOS, and 2 cases were of AML, MRC. In AML, RGA, APL with PML-RARA positive cases were 10 out of 20 cases, AML with (8;21) RUNX1-RUNX1T1 were 7/20 cases; there were two cases of AML with mutated NPM1 gene and one case of AML with biallelic mutation of CEBPA. In AML, NOS subcategory AML with maturation was more common with 9/21cases. In subcategory of ALL, B-ALL was more common than T-ALL. B-ALL, NOS was more common than B-ALL, RGA and we had 1 case of NK cell Leukemia.
The application of revised 4th edition WHO 2016 classification confers uniformity in reporting acute leukemia cases that aids in the treatment by using targeted therapies and helps in the prediction of prognosis. The WHO classification for acute leukemias is very objective, therapy oriented and the need of the hour.
急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）是血液恶性肿瘤中具有临床和生物学多样性表型的疾病。本研究的目的是根据 2016 年版 WHO 修订版第 4 版对 AL 进行诊断和分类，并研究其临床病理特征。
这项横断面观察性研究纳入了 68 例被诊断为 AL 的患者。诊断基于外周血涂片检查、骨髓穿刺、流式细胞术以及细胞遗传学和分子研究。
在 2 年的时间里诊断出 68 例 AL 病例，其中 25 例为 ALL，43 例为 AML。根据 2016 年版 WHO 进行的 AML 亚分类中，20 例为 AML、RGA，21 例为 AML、NOS，2 例为 AML、MRC。在 AML、RGA 中，APL 伴 PML-RARA 阳性的病例为 20 例中的 10 例，AML 伴（8；21）RUNX1-RUNX1T1 的病例为 20 例中的 7 例；有 2 例 AML 伴 NPM1 基因突变，1 例 AML 伴 CEBPA 双等位基因突变。在 AML、NOS 亚类中，伴成熟的 AML 更为常见，共 21 例中的 9 例。在 ALL 亚类中，B-ALL 比 T-ALL 更为常见。B-ALL、NOS 比 B-ALL、RGA 更为常见，我们有 1 例 NK 细胞白血病。
应用修订后的 2016 年版 WHO 第 4 版分类在报告急性白血病病例方面具有一致性，有助于通过靶向治疗进行治疗，并有助于预测预后。WHO 对急性白血病的分类非常客观、面向治疗，是当前的需要。
