利用污水追踪新冠病毒，了解无症状传播。
Tracking COVID-19 with wastewater to understand asymptomatic transmission.
机构信息
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; School of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia.
Laboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa, Japan.
出版信息
Int J Infect Dis. 2021 Jul;108:296-299. doi: 10.1016/j.ijid.2021.05.005. Epub 2021 May 11.
INTRODUCTION
SARS-CoV-2 RNA is excreted in feces of most patients, therefore viral load in wastewater can be used as a surveillance tool to develop an early warning system to help and manage future pandemics.
METHODS
We collected wastewater from 24 random locations at Bangkok city center and 26 nearby suburbs from July to December 2020. SARS-CoV-2 RNA copy numbers were measured using real-time polymerase chain reaction (PCR).
RESULTS
SARS-CoV-2 RNA was detected in wastewater from both the city center and suburbs. Except for July, there were no significant differences in copy numbers between the city center and suburbs. Between October and November, a sharp rise in copy number was observed in both places followed by two to three times increase in December, related to SARS-CoV-2 cases reported for same month.
CONCLUSIONS
Our study provided the first dataset related to SARS-CoV-2 viral RNA in the wastewater of Bangkok. Our results suggest that wastewater could be used as a complementary source for detecting viral RNA and predicting upcoming outbreaks and waves.
简介
大多数患者的粪便中都会排出 SARS-CoV-2 RNA，因此废水中的病毒载量可作为一种监测工具，用于建立早期预警系统，以帮助和管理未来的大流行。
方法
我们于 2020 年 7 月至 12 月从曼谷市中心的 24 个随机地点和 26 个附近郊区采集了废水。使用实时聚合酶链反应（PCR）来测量 SARS-CoV-2 RNA 拷贝数。
结果
在市中心和郊区的废水中均检测到了 SARS-CoV-2 RNA。除了 7 月，市中心和郊区之间的拷贝数没有明显差异。10 月至 11 月，两个地方的拷贝数均急剧上升，随后 12 月增加了两到三倍，与当月报告的 SARS-CoV-2 病例有关。
结论
我们的研究提供了有关曼谷废水的 SARS-CoV-2 病毒 RNA 的首个数据集。我们的结果表明，废水可作为检测病毒 RNA 和预测即将到来的暴发和波次的补充来源。
Zotero 插件