Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Sci Total Environ. 2021 Sep 15;787:147510. doi: 10.1016/j.scitotenv.2021.147510. Epub 2021 May 5.
With the increasing production and wide application of carbon nanotubes (CNTs), they are inevitably released into the natural environment and ecosystems, where plants are the main primary producers. Hence, it is imperative to understand the toxic effects of CNTs on plants. The molecular mechanisms underlying the toxic effects of CNTs on plants are still unclear. Therefore, in the present study, we investigated the effects of high concentrations of multi-walled CNTs (MWCNTs) on Arabidopsis. Root elongation and leaf development were severely inhibited after MWCNT exposure. Excess production of HO, O, and malondialdehyde was observed, indicating that MWCNTs induced oxidative stress. The antioxidant system was activated to counter MWCNTs-induced oxidative stress. Combinatorial transcriptome and m6A methylome analysis revealed that MWCNTs suppressed auxin signaling and photosynthesis. Reactive oxygen species metabolism, toxin metabolism, and plant responses to pathogens were enhanced to cope with the phytotoxicity of MWCNTs. Our results provide new insights into the molecular mechanisms of CNT phytotoxicity and plant defense responses to CNTs.
随着碳纳米管（CNTs）的产量增加和广泛应用，它们不可避免地会释放到自然环境和生态系统中，而植物是主要的初级生产者。因此，了解 CNTs 对植物的毒性作用至关重要。CNTs 对植物毒性作用的分子机制尚不清楚。因此，在本研究中，我们研究了高浓度多壁 CNTs（MWCNTs）对拟南芥的影响。MWCNT 暴露后，根伸长和叶片发育受到严重抑制。过量的 HO、O 和丙二醛的产生表明 MWCNTs 诱导了氧化应激。抗氧化系统被激活以对抗 MWCNTs 诱导的氧化应激。组合转录组和 m6A 甲基组分析表明，MWCNTs 抑制了生长素信号和光合作用。为了应对 MWCNTs 的植物毒性，活性氧代谢、毒素代谢和植物对病原体的反应增强。我们的研究结果为 CNT 植物毒性的分子机制和植物对 CNTs 的防御反应提供了新的见解。
