Cao Runyu, Xiao Wen, Pan Feng, Tian Ran, Wu Xintong, Sun Lianwen
Key Laboratory of Precision Opto-mechatronics Technology, School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100083, China.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, China.
Biomed Opt Express. 2021 Mar 9;12(4):1922-1933. doi: 10.1364/BOE.418418. eCollection 2021 Apr 1.
Osteocytes, as the mechano-sensors in bone, are always subjected to fluid shear stress (FSS) from the surrounding matrix. Quantification of FSS-induced cellular deformation is significant for clarifying the "perceive and transmit" process of cellular mechanotransduction. In this research, a label-free displacement and strain mapping method based on digital holographic microscopy (DHM) and digital image correlation (DIC) is introduced. The method, which is termed DHM-DIC, innovatively utilizes surface features extracted from holographic phase images instead of speckles as the metric for DIC searching. Simulation results on a hemisphere validate the feasibility of DHM-DIC. Displacement and strain maps of living osteocytes under 1.5 Pa FSS are evaluated from DHM-DIC and present good agreement with our previous finite element modeling results.
骨细胞作为骨骼中的机械传感器，始终受到来自周围基质的流体剪切应力（FSS）作用。量化FSS诱导的细胞变形对于阐明细胞机械转导的“感知与传递”过程具有重要意义。在本研究中，引入了一种基于数字全息显微镜（DHM）和数字图像相关（DIC）的无标记位移和应变映射方法。该方法被称为DHM-DIC，创新性地利用从全息相位图像中提取的表面特征而非散斑作为DIC搜索的度量标准。在半球上的模拟结果验证了DHM-DIC的可行性。通过DHM-DIC评估了1.5 Pa FSS作用下活骨细胞的位移和应变图，其结果与我们之前的有限元建模结果吻合良好。
