Asif Abuzar A, Roy Moni, Tellier Benjamin R, Ahmad Sharjeel
Department of Internal Medicine, University of Illinois College of Medicine, Peoria, OSF Saint Francis Medical Center, 530 NE Glen Oak Ave, Peoria, IL 61637, USA.
Section of Infectious Diseases, University of Illinois College of Medicine, Peoria, OSF Saint Francis Medical Center, 530 NE Glen Oak Ave, Peoria, IL 61637, USA.
IDCases. 2021 Apr 20;24:e01126. doi: 10.1016/j.idcr.2021.e01126. eCollection 2021.
meningitis is frequently caused by exposure to dog or cat bites and occurs more commonly in immunocompromised individuals. CSF analysis is the first step in diagnosis; however, in situations where CSF cultures turn negative, molecular techniques such as 16S rRNA gene amplification followed by polymerase chain reaction (PCR) product sequencing have shown promise. Next generation sequencing of cell free DNA (NGS cfDNA) can assist in identifying the causative agent in a quick and accurate manner. We present a rare case of meningitis in an immunocompetent host that highlights the utility of NGS cfDNA in timely diagnosis after exhausting all other available diagnostic techniques.
脑膜炎常由接触狗或猫的咬伤引起，在免疫功能低下的个体中更为常见。脑脊液分析是诊断的第一步；然而，在脑脊液培养结果为阴性的情况下，诸如16S rRNA基因扩增后进行聚合酶链反应（PCR）产物测序等分子技术已显示出前景。游离DNA的下一代测序（NGS cfDNA）可以帮助快速准确地识别病原体。我们报告一例免疫功能正常宿主患脑膜炎的罕见病例，该病例突出了在穷尽所有其他可用诊断技术后，NGS cfDNA在及时诊断中的作用。
