Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, United States.
Department of Biomedical Engineering, University of Connecticut, Storrs, CT, United States.
Front Cell Infect Microbiol. 2021 Apr 30;11:663949. doi: 10.3389/fcimb.2021.663949. eCollection 2021.
As the COVID-19 pandemic continues, people are becoming infected at an alarming rate, individuals are unknowingly spreading disease, and more lives are lost every day. There is an immediate need for a simple, rapid, early and sensitive point-of-care testing for COVID-19 disease. However, current testing approaches do not meet such need. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods have received substantial attention for nucleic acid-based molecular testing due to their simplicity, high sensitivity and specificity. This review explores the various CRISPR-based COVID-19 detection methods and related diagnostic devices. As with any emerging technology, CRISPR/Cas-based nucleic acid testing methods have several challenges that must be overcome for practical applications in clinics and hospitals. More importantly, these detection methods are not limited to COVID-19 but can be applied to detect any type of pathogen, virus, and fungi that may threaten humans, agriculture, and food industries in resource-limited settings. CRISPR/Cas-based detection methods have the potential to become simpler, more reliable, more affordable, and faster in the near future, which is highly important for achieving point-of-care diagnostics.
随着 COVID-19 大流行的持续，人们以惊人的速度被感染，个体在不知不觉中传播疾病，每天都有更多的生命逝去。目前急需一种简单、快速、早期和敏感的 COVID-19 疾病床边即时检测方法。然而，目前的检测方法无法满足这一需求。最近，基于成簇规律间隔短回文重复序列（CRISPR）的检测方法因其简单、高灵敏度和特异性而受到基于核酸的分子检测的广泛关注。本综述探讨了各种基于 CRISPR 的 COVID-19 检测方法和相关诊断设备。与任何新兴技术一样，基于 CRISPR/Cas 的核酸检测方法在临床和医院的实际应用中存在一些必须克服的挑战。更重要的是，这些检测方法不仅限于 COVID-19，还可以应用于检测在资源有限的环境中可能威胁人类、农业和食品工业的任何类型的病原体、病毒和真菌。在不久的将来，基于 CRISPR/Cas 的检测方法有可能变得更简单、更可靠、更经济实惠、更快，这对于实现床边即时诊断非常重要。
