Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China.
J Anim Sci. 2019 Apr 3;97(4):1745-1756. doi: 10.1093/jas/skz057.
This study was conducted to evaluate the effects of dietary resveratrol supplementation on growth performance, meat quality, serum lipid profiles, intramuscular fat (IMF) deposition, and the expression levels of several lipid metabolism-related miRNAs and genes in growing-finishing pigs. A total of 36 healthy crossbred pigs (Duroc × Landrace × Yorkshire) with an average initial BW of 24.67 ± 3.49 kg were randomly divided into two groups and fed either with a basal diet (CON) or basal diet containing 600 mg/kg resveratrol (RES). The trial lasted for 119 d. Resveratrol had no significant effect on growth performance and carcass characteristics. However, the concentrations of serum triglyceride, total cholesterol, low-density lipoprotein cholesterol, and very low-density lipoprotein were lower in RES group than those of CON group (P < 0.05). Dietary resveratrol supplementation increased the IMF content in longissimus dorsi (P < 0.05), up-regulated mRNA abundances of peroxisome proliferator-activated receptor γ, fatty acid synthase, acetyl-CoA carboxylase, and lipoprotein lipase (P < 0.05), while downregulated mRNA abundances of carnitine palmitoyl transferase-1, sirtuin 1, and peroxisome proliferator-activated receptor α (P < 0.05) in LM. In addition, resveratrol enhanced (P < 0.05) the expression of ssc-miR-181a, ssc-miR-370, and ssc-miR-21 and reduced (P < 0.05) the expression of ssc-miR-27a in longissimus dorsi. These results indicated that dietary resveratrol supplementation significantly improved IMF content and decreased serum lipids levels, which might be related with the changes in ssc-miR-181a, ssc-miR-370, ssc-miR-21, ssc-miR-27a and their downstream genes expression.
这项研究旨在评估膳食白藜芦醇补充对生长性能、肉质、血清脂质谱、肌内脂肪（IMF）沉积以及生长肥育猪中几种脂质代谢相关 miRNA 和基因表达水平的影响。总共选择了 36 头平均初始体重为 24.67 ± 3.49kg 的健康杂交猪（杜洛克×长白×约克夏），并将其随机分为两组，分别饲喂基础日粮（CON）或基础日粮添加 600mg/kg 白藜芦醇（RES）。试验持续 119 天。白藜芦醇对生长性能和胴体特性没有显著影响。然而，RES 组血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇的浓度均低于 CON 组（P＜0.05）。饲粮添加白藜芦醇增加了背最长肌的 IMF 含量（P＜0.05），上调了过氧化物酶体增殖物激活受体γ、脂肪酸合酶、乙酰辅酶 A 羧化酶和脂蛋白脂肪酶的 mRNA 丰度（P＜0.05），下调了肉碱棕榈酰转移酶-1、Sirtuin 1 和过氧化物酶体增殖物激活受体α的 mRNA 丰度（P＜0.05）。此外，白藜芦醇增强了背最长肌中 ssc-miR-181a、ssc-miR-370 和 ssc-miR-21 的表达（P＜0.05），降低了 ssc-miR-27a 的表达（P＜0.05）。这些结果表明，饲粮白藜芦醇补充显著提高了 IMF 含量，降低了血清脂质水平，这可能与 ssc-miR-181a、ssc-miR-370、ssc-miR-21 和它们下游基因表达的变化有关。
