Ma Yanfen, Feng Ying, Song Liwen, Li Muyang, Dai Hongyu, Bao Hua, Zhang Guijie, Zhao Lei, Zhang Chunhua, Yi Jing, Liang Yusheng
School of Agriculture, Ningxia University, Yinchuan 750021, China.
Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China.
Anim Nutr. 2021 Mar;7(1):206-215. doi: 10.1016/j.aninu.2020.06.005. Epub 2020 Dec 19.
Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; = 50) or control (without GTP; = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.
围产期奶牛常出现能量负平衡，因此易患高酮血症等代谢疾病，这会给奶牛场造成经济损失。本研究旨在探讨围产期补充绿茶多酚（GTP）对患有高酮血症的奶牛生产性能、氧化应激和免疫代谢的影响。根据预期分娩前15天的体重、既往产奶量和胎次，将100头荷斯坦奶牛分为GTP组（0.2 g/kg干物质；n = 50）或对照组（不补充GTP；n = 50）。随后，根据产后2至3天血液β-羟基丁酸（BHBA）浓度在1.2至2.9 mmol/L之间，从每组中选出10头患有高酮血症的奶牛。所有奶牛从产前15天到产后30天，饲喂含或不含GTP的围产前期日粮和泌乳日粮。在产后10、20和30天，从20头患有高酮血症的奶牛中采集牛奶和血液样本。与对照奶牛相比，GTP组奶牛产奶量更高，体细胞数更低。GTP组的BHBA、游离脂肪酸、胆固醇、甘油三酯、活性氧、丙二醛和过氧化氢浓度较低，葡萄糖浓度较高，天冬氨酸转氨酶、丙氨酸转氨酶和谷氨酰转肽酶活性较低，同时超氧化物歧化酶、谷胱甘肽过氧化物酶活性和总抗氧化能力较高。此外，补充GTP上调了血浆中白细胞介素-6和白细胞介素-10的浓度，但下调了肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-2、白细胞介素-8和干扰素-γ的浓度。GTP组血浆免疫球蛋白G浓度也更高。总体而言，数据表明，从产前15天到产后30天补充GTP可提高患有高酮血症的奶牛在泌乳早期的产奶量和健康状况。
