Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
J Clin Periodontol. 2021 Sep;48(9):1250-1259. doi: 10.1111/jcpe.13479. Epub 2021 Jun 20.
To explore the feasibility of screening for periodontitis by measuring biomarkers, namely total proteolytic activity (TPA), matrix metalloproteinase (MMP)-8, chitinase, lysozyme or their combination, in saliva, oral rinse and gingival crevicular fluid (GCF).
Subjects were recruited among healthy/gingivitis individuals and untreated periodontitis patients in Academic Centre for Dentistry Amsterdam (ACTA). All participants donated samples of unstimulated whole saliva, oral rinse and GCF. The protein concentrations and MMP-8 levels were determined by ELISA. Enzymatic activities were measured using appropriate fluorogenic substrates.
In oral rinse samples, periodontitis patients (n = 19) exhibited significantly higher concentrations of MMP-8 and TPA than controls (n = 20). MMP-8 in combination with chitinase explained 88% of the variance and assigned a subject to control or periodontitis group, with best accuracy (87.2%) in oral rinse.
The combination of MMP-8 and chitinase in the current oral rinse procedure has the potential to discriminate periodontitis from periodontal health/gingivitis.
通过测量唾液、漱口液和龈沟液中的生物标志物（即总蛋白水解活性（TPA）、基质金属蛋白酶（MMP）-8、几丁质酶、溶菌酶或其组合）来探索牙周炎筛查的可行性。
在阿姆斯特丹牙科学术中心（ACTA），从健康/牙龈炎个体和未经治疗的牙周炎患者中招募受试者。所有参与者均捐献了未刺激的全唾液、漱口液和龈沟液样本。通过 ELISA 测定蛋白浓度和 MMP-8 水平。使用适当的荧光底物测定酶活性。
在漱口液样本中，牙周炎患者（n=19）的 MMP-8 和 TPA 浓度明显高于对照组（n=20）。MMP-8 与几丁质酶联合可解释 88%的方差，并将受试者分配到对照组或牙周炎组，在漱口液中具有最佳的准确性（87.2%）。
当前漱口液程序中 MMP-8 和几丁质酶的联合具有区分牙周炎与牙周健康/牙龈炎的潜力。
