黏蛋白 4 和基质金属蛋白酶 7 作为牙周炎的新型唾液生物标志物。
Mucin 4 and matrix metalloproteinase 7 as novel salivary biomarkers for periodontitis.
机构信息
Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, Huddinge, Sweden.
Department of Periodontology, Folktandvården Eastmaninstitutet, Stockholm, Sweden.
出版信息
J Clin Periodontol. 2017 Mar;44(3):247-254. doi: 10.1111/jcpe.12670. Epub 2017 Feb 6.
AIM
Periodontitis is a chronic inflammatory disease, characterized by irreversible destruction of tooth-supporting tissue including alveolar bone. We recently reported mucin 4 (MUC4) and matrix metalloproteinase 7 (MMP7) as highly associated with periodontitis in gingival tissue biopsies. The aim of this study was to further investigate the levels of MUC4 and MMP7 in saliva and gingival crevicular fluid (GCF) samples of patients with periodontitis.
MATERIALS AND METHODS
Saliva and GCF samples were collected from periodontitis patients and healthy controls. The levels of MUC4, MMP7, and total protein concentrations were analysed using ELISA or Bradford assay.
RESULTS
MUC4 levels were significantly lower in saliva and GCF from periodontitis patients relative to healthy controls. MMP7 levels were significantly higher in saliva and GCF from periodontitis patients. Multivariate analysis revealed that MUC4 was significantly associated with periodontitis after adjusting for age and smoking habits and, moreover, that the combination of MUC4 and MMP7 accurately discriminated periodontitis from healthy controls.
CONCLUSIONS
MUC4 and MMP7 may be utilized as possible novel biomarkers for periodontitis.
目的
牙周炎是一种慢性炎症性疾病，其特征是包括牙槽骨在内的牙齿支持组织的不可逆破坏。我们最近报道黏蛋白 4（MUC4）和基质金属蛋白酶 7（MMP7）与牙龈组织活检中的牙周炎高度相关。本研究旨在进一步研究牙周炎患者唾液和龈沟液（GCF）样本中 MUC4 和 MMP7 的水平。
材料和方法
收集牙周炎患者和健康对照组的唾液和 GCF 样本。使用 ELISA 或 Bradford 测定法分析 MUC4、MMP7 和总蛋白浓度。
结果
与健康对照组相比，牙周炎患者的唾液和 GCF 中的 MUC4 水平显著降低。牙周炎患者的唾液和 GCF 中的 MMP7 水平显著升高。多变量分析显示，在调整年龄和吸烟习惯后，MUC4 与牙周炎显著相关，此外，MUC4 和 MMP7 的组合可以准确地区分牙周炎与健康对照组。
结论
MUC4 和 MMP7 可用作牙周炎的潜在新型生物标志物。
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