Dwitiyanti Dwitiyanti, Harahap Yahdiana, Elya Berna, Bahtiar Anton
Universitas Indonesia, Kampus UI, Depok 16424, West Java, Indonesia.
Department of Bioanalysis, Universitas Indonesia, Kampus UI, Depok 16424, West Java, Indonesia.
Adv Pharmacol Pharm Sci. 2021 Apr 28;2021:8869571. doi: 10.1155/2021/8869571. eCollection 2021.
Patients with diabetes are 1.6 times more likely to use complementary alternative medicine than nondiabetic patients. Previous studies have shown that (Tenore) Steen. () leaf extract has the capacity to lower blood glucose, but the actual mechanisms are unclear. Therefore, in this study, we explored the effect of leaf extract on the metabolism of fatty acids and amino acids. Six-week-old male Wistar rats were randomly divided into six experimental groups ( = 5 per group). Two groups were fed with a regular diet or a high-fat diet (HFD) for six weeks. The regular diet and HFD groups were administered with 0.5% carboxymethylcellulose as a vehicle, and HFD rats were also fed with a suspension of glibenclamide (0.51 mg/kg body weight (BW)) or leaf extract (25, 50, and 100 mg/kg BW). During the whole treatment, BW and food intake were recorded weekly. The rats were euthanized seven weeks after treatment. Blood glucose was evaluated by spectrophotometry, while fatty acids and amino acids were evaluated using a gas chromatography/flame ionization detector (GC/FID). All doses of administration reduced blood glucose significantly, and 50 mg/kg BW was most effective in lowering blood glucose, similar to the effects of glibenclamide. leaf extract affected the levels of medium-chain fatty acids, especially at 50 mg/kg BW. In contrast, glibenclamide affected long-chain fatty acids (LCFAs) to lower blood glucose. Based on the analysis conducted, we conclude that administration of leaf extract can decrease blood glucose levels by regulating fatty acid metabolism and that a dose of 50 mg/kg BW in rats was the optimal dose.
糖尿病患者使用补充替代医学的可能性是非糖尿病患者的1.6倍。先前的研究表明，（Tenore）Steen.（）叶提取物具有降低血糖的能力，但实际机制尚不清楚。因此，在本研究中，我们探讨了叶提取物对脂肪酸和氨基酸代谢的影响。将六周龄雄性Wistar大鼠随机分为六个实验组（每组n = 5）。两组分别喂食常规饮食或高脂饮食（HFD）六周。常规饮食组和HFD组给予0.5%羧甲基纤维素作为赋形剂，HFD组大鼠还喂食格列本脲悬浮液（0.51 mg/kg体重（BW））或叶提取物（25、50和100 mg/kg BW）。在整个治疗过程中，每周记录体重和食物摄入量。治疗七周后对大鼠实施安乐死。通过分光光度法评估血糖，使用气相色谱/火焰离子化检测器（GC/FID）评估脂肪酸和氨基酸。所有剂量的给药均显著降低血糖，50 mg/kg BW在降低血糖方面最有效，与格列本脲的效果相似。叶提取物影响中链脂肪酸水平，尤其是在50 mg/kg BW时。相比之下，格列本脲影响长链脂肪酸（LCFA）以降低血糖。基于所进行的分析，我们得出结论，给予叶提取物可通过调节脂肪酸代谢降低血糖水平，并且在大鼠中50 mg/kg BW的剂量是最佳剂量。
