短乳杆菌KU15153对致龋变形链球菌KCTC 5316的抑制作用。
Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries.
作者信息
Jang Hye Ji, Kim Jong Ha, Lee Na-Kyoung, Paik Hyun-Dong
机构信息
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, South Korea.
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, South Korea.
出版信息
Microb Pathog. 2021 Aug;157:104938. doi: 10.1016/j.micpath.2021.104938. Epub 2021 May 20.
This study determined the inhibitory effect of Lactobacillus brevis KU15153 against cariogenic Streptococcus mutans KCTC 5316. Antimicrobial activity, auto-aggregation, cell surface hydrophobicity, exopolysaccharides (EPS) production, biofilm formation, and morphological changes were assessed in the presence of L. brevis KU15153. L. brevis KU15153 exhibited the highest antimicrobial activity against S. mutans KCTC 5316 (28.67 ± 4.16 mm). Auto-aggregation (38.32%), cell surface hydrophobicity (27.08%), and EPS production rate (58.52%) of S. mutans KCTC 5316 slightly decreased upon treatment with L. brevis KU15153. Additionally, crystal violet stanning and scanning electron microscopy confirmed the L. brevis KU15153-mediated inhibition of biofilm formation by S. mutans KCTC 5316 in comparison to that observed in the negative control (untreated S. mutans KCTC 5316). These results indicate that the L. brevis KU15153 could be used as a potential probiotic for maintaining oral health.
本研究确定了短乳杆菌KU15153对致龋变形链球菌KCTC 5316的抑制作用。在短乳杆菌KU15153存在的情况下，评估了其抗菌活性、自聚集、细胞表面疏水性、胞外多糖（EPS）产生、生物膜形成及形态变化。短乳杆菌KU15153对变形链球菌KCTC 5316表现出最高的抗菌活性（28.67±4.16毫米）。用短乳杆菌KU15153处理后，变形链球菌KCTC 5316的自聚集（38.32%）、细胞表面疏水性（27.08%）和EPS产生率（58.52%）略有下降。此外，与阴性对照（未处理的变形链球菌KCTC 5316）相比，结晶紫染色和扫描电子显微镜证实了短乳杆菌KU15153介导的对变形链球菌KCTC 5316生物膜形成的抑制作用。这些结果表明，短乳杆菌KU15153可作为维持口腔健康的潜在益生菌。
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