Laboratory of Applied Microbiology, Department of Health Sciences (DiSS), Center for Translational Research on Allergic and Autoimmune Diseases (CAAD), School of Medicine, Università del Piemonte Orientale (UPO), Corso Trieste 15/A, 28100 Novara, Italy.
Clinical Chemistry Unit, DiSS, School of Medicine, University Hospital "Maggiore della Carità", Università del Piemonte Orientale (UPO), Corso Mazzini 18, 28100 Novara, Italy.
Int J Mol Sci. 2022 Dec 18;23(24):16163. doi: 10.3390/ijms232416163.
The human oral pathobionts , and , in dysbiosis-promoting conditions, lead to oral infections, which also represent a threat to human systemic health. This scenario may be worsened by antibiotic misuse, which favours multi-drug resistance, making the research on pathogen containment strategies more than crucial. Therefore, we aimed to in vitro select the most promising probiotic strains against oral pathogen growth, viability, biofilm formation, and co-aggregation capacity, employing both the viable probiotics and their cell-free supernatants (CFSs). Interestingly, we also assessed probiotic efficacy against the three-pathogen co-culture, mimicking an environment similar to that in vivo. Overall, the results showed that CFSs performed better than the , highlighting LRE11, LR04, LC04, and LF26 as the most effective strains, opening the chance to deeper investigation of their action and CFS composition. Altogether, the methodologies presented in this study can be used for probiotic efficacy screenings, in order to better focus the research on a viable probiotic, or on its postbiotics, suitable in case of infections.
人类口腔共生菌在促进失调的条件下，会导致口腔感染，这也对人类全身健康构成威胁。这种情况可能因抗生素滥用而恶化，抗生素滥用有利于多重耐药性的产生，这使得研究病原体控制策略变得至关重要。因此，我们旨在体外选择最有前途的益生菌菌株，以抑制口腔病原体的生长、活力、生物膜形成和共聚能力，同时使用活菌和其无细胞上清液（CFS）。有趣的是，我们还评估了益生菌对三病原体共培养的功效，模拟体内类似的环境。总的来说，结果表明 CFS 比活菌表现更好，突出了 LRE11、LR04、LC04 和 LF26 作为最有效的菌株，为进一步研究它们的作用和 CFS 组成开辟了机会。总之，本研究中提出的方法可用于益生菌功效筛选，以便更好地将研究重点放在有活力的益生菌或其后生元上，以便在感染时使用。
