Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea.
Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan 31538, Republic of Korea.
J Microbiol Biotechnol. 2021 Jul 28;31(7):999-1010. doi: 10.4014/jmb.2104.04023.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment. They are highly toxigenic and carcinogenic. Probiotic bacteria isolated from fermented foods were tested to check their ability to degrade and/or detoxify PAHs. Five probiotic bacteria with distinct morphologies were isolated from a mixture of 26 fermented foods co-cultured with benzo(a)pyrene (BaP) containing Bushnell Haas minimal broth. Among them, (PMC10) significantly reduced the abundance of BaP in the broth. PMC10 completely degraded BaP presented at a lower concentration in broth culture. also showed a clear zone of degradation on a BaP-coated Bushnell Haas agar plate. Gene expression profiling showed significant increases of PAH ringhydroxylating dioxygenases and 4-hydroxybenzoate 3-monooxygenase genes in in response to BaP treatment. In addtion, both live and heat-killed removed BaP and naphthalene (Nap) from phosphate buffer solution. Live did not show any cytotoxicity to macrophage or human dermal fibroblast cells. Live-cell and cell-free supernatant of showed potential anti-inflammatory effects. Cell-free supernatant and extract of also showed free radical scavenging effects. These results highlight the prospective ability of to biodegrade and remove toxic PAHs from the human body and suggest that the biodegradation of BaP might be regulated by ring-hydroxylating dioxygenase-initiated metabolic pathway.
多环芳烃（PAHs）广泛存在于环境中。它们具有高度的毒性和致癌性。从发酵食品中分离出的益生菌被测试以检查其降解和/或解毒 PAHs 的能力。从含有苯并(a)芘（BaP）的 Bushnell Haas 最小肉汤与 26 种发酵食品混合物中分离出五种具有不同形态的益生菌。其中，（PMC10）显著降低了肉汤中 BaP 的丰度。PMC10 完全降解了肉汤培养物中较低浓度的 BaP。在涂有 BaP 的 Bushnell Haas 琼脂平板上，也显示出明显的降解区。基因表达谱分析显示，在 BaP 处理下， 中 PAH 环羟化双加氧酶和 4-羟基苯甲酸 3-单加氧酶基因的表达显著增加。此外，活菌和热灭活的 从磷酸盐缓冲溶液中去除了 BaP 和萘（Nap）。活菌对巨噬细胞或人真皮成纤维细胞没有显示出任何细胞毒性。活菌和细胞上清液均显示出潜在的抗炎作用。 细胞上清液和提取物也显示出自由基清除作用。这些结果突出了 降解和从人体中去除有毒 PAHs 的潜力，并表明 BaP 的生物降解可能受环羟化双加氧酶启动的代谢途径调节。
