Medicinal Aromatic and Economic Plants Group, Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam, India.
AcSIR-Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India.
J Food Biochem. 2021 Jul;45(7):e13767. doi: 10.1111/jfbc.13767. Epub 2021 May 24.
Callistemon citrinus aerial part essential oil (CCEO) was analyzed for chemical compositions using GC/MS. Pharmacological activities such as neurodegenerative inhibitory, antioxidant, anti-inflammatory, antimicrobial, and acetylcholinesterase activities were evaluated using the standard methodologies. Genotoxicity was investigated using Allium cepa assay. GC/MS analysis identified 27 compounds; eucalyptol (55.40%) was the major component. Radical scavenging activity showed IC value of 16.71 μg/mL. Protein denaturation assay showed IC value of 21.19 μg/mL and 19.53μg/mL in protease inhibitor activity. MIC assay revealed antimicrobial potential of CCEO against microbial strains B. cereus at 2.00 mg/mL, S. typhimurium at 4.50 mg/mL, S. mutans at 2.50 mg/mL, C. albicans at 4.00 mg/mL, and S. cerevisiae at 4.50 mg/mL concentrations. Mitotic index value (MI) of CCEO showed negligible genotoxicity with MI 17.25% close to distilled water 18.22%. Acetylcholinesterase strong inhibitory activity of CCEO was observed from IC = 6.335 μg/mL. CCEO could be a cheap and easy source for the extraction of the pure compound eucalyptol and possess various biological activities which increase its pharmacological value as well as its applicability in the field of flavor and fragrance industries. PRACTICAL APPLICATIONS: Eucalyptol is the major component of many pharmaceutical and food/flavor industries. The present investigation provides a new source for the isolation of pure compound eucalyptol in cost-effective way. Additionally, the essential oil could also be used for the pharmaceutical formulation of antioxidant, inflammation inhibitory, and neurodegenerative inhibitory drug preparation under the safety range concentration.
柠檬桉空中部分精油（CCEO）使用 GC/MS 进行化学成分分析。使用标准方法评估神经退行性抑制、抗氧化、抗炎、抗菌和乙酰胆碱酯酶活性等药理活性。使用 Allium cepa 测定法研究遗传毒性。GC/MS 分析鉴定出 27 种化合物；桉油醇（55.40%）为主要成分。自由基清除活性的 IC 值为 16.71μg/mL。蛋白变性试验显示蛋白酶抑制剂活性的 IC 值分别为 21.19μg/mL 和 19.53μg/mL。MIC 测定法显示 CCEO 对微生物菌株 B. cereus 的抗菌潜力为 2.00mg/mL，对 S. typhimurium 的抗菌潜力为 4.50mg/mL，对 S. mutans 的抗菌潜力为 2.50mg/mL，对 C. albicans 的抗菌潜力为 4.00mg/mL，对 S. cerevisiae 的抗菌潜力为 4.50mg/mL。CCEO 的有丝分裂指数值（MI）显示出可忽略的遗传毒性，MI 为 17.25%，接近蒸馏水的 18.22%。CCEO 对乙酰胆碱酯酶具有很强的抑制活性，IC 值为 6.335μg/mL。CCEO 可以作为提取纯桉油醇的廉价易得的来源，并且具有多种生物活性，这增加了其药理学价值以及在香料和香精行业的适用性。实际应用：桉油醇是许多制药和食品/香料工业的主要成分。本研究提供了一种从成本效益高的方式中分离纯桉油醇的新来源。此外，在安全浓度范围内，该精油还可用于制备抗氧化、抗炎和神经退行性抑制药物的药物制剂。
