State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
J Agric Food Chem. 2021 Jun 9;69(22):6229-6239. doi: 10.1021/acs.jafc.1c00869. Epub 2021 May 24.
(Zingiberaceae) has been an important food spice and vegetable in southern China. Fifteen phenolic compounds (-) including three new diarylheptanoids, rhynchanines A-C (-) and one new phenylpropanoid, 4--methylstroside B (), were isolated from rhizomes. The structures of new compounds were elucidated by comprehensive analyses through NMR, HRMS technique, acid hydrolysis, and Mosher's reaction. Among them, compound is the first isolated natural product and its NMR data are reported. Most of the isolated compounds, especially - and , showed significant antioxidant activities on DPPH, ABTS radical scavenging, and FRAP assays. Furthermore, the antioxidant phenolic compounds were evaluated for their cytoprotective capacity against HO-induced oxidative stress in HepG-2 cells. Compounds and could significantly inhibit reactive oxygen species production, and compounds , , and could remarkably prevent the cell apoptosis. Then, the rhizome, which contained phenolic compounds, might serve as a functional food for potential application on preventing oxidative stress-connected diseases.
（姜科）一直是中国南方重要的食用香料和蔬菜。从根茎中分离得到了 15 种酚类化合物（-），包括三种新的二芳基庚烷类化合物 rhynchanines A-C（-）和一种新的苯丙素类化合物 4--甲基 stroside B（）。通过 NMR、高分辨质谱技术、酸水解和 Mosher 反应的综合分析，确定了新化合物的结构。其中，化合物 是第一个分离得到的天然产物，并报道了其 NMR 数据。分离得到的大多数化合物，特别是 - 和 ，在 DPPH、ABTS 自由基清除和 FRAP 测定中表现出显著的抗氧化活性。此外，还评估了抗氧化酚类化合物对 HepG-2 细胞中 HO 诱导的氧化应激的细胞保护能力。化合物 和 能显著抑制活性氧的产生，化合物 、 、 能显著防止细胞凋亡。因此，含有酚类化合物的根茎可能作为一种功能性食品，具有预防与氧化应激相关疾病的应用潜力。
