Student Research Committee, Department of Molecular Medicine, Faculty of medicine, Birjand University of Medical Sciences, Birjand, Iran.
Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
J Clin Lab Anal. 2021 Jun;35(6):e23770. doi: 10.1002/jcla.23770. Epub 2021 May 24.
The efficiency of high-density lipoprotein (HDL) to efflux cholesterol contributes to the reverse cholesterol transport (RCT) pathway as one of HDL's proposed functions and depends on the ability of HDL to uptake cholesterol. We aimed to investigate cholesterol uptake capacity (CUC) by a newly developed assay in samples from the MASHAD (Mashhad Stroke and Heart Atherosclerotic Disorders) cohort study.
The study population comprised 153 individuals developed CVD diagnosed by a specialist cardiologist, over 6 years of follow-up, and 350 subjects without CVD. We used a modified CUC method to evaluate the functionality of HDL in serum samples.
The CUC assay was highly reproducible with values for inter- and intra-assay variation of 13.07 and 6.65, respectively. The mean serum CUC was significantly lower in the CVD group compared to control (p = 0.01). Although, there were no significant differences in serum HDL-C between the groups and there was no significantly association with risk of progressive CVD. Multivariate logistic regression analysis showed that there was a significantly negative association between CUC and risk of CVD after adjustment for confounding parameters (OR = 0.57, 95% CI = 0.38-0.87, p = 0.009). The CUC was also inversely and independently associated with the risk of CVD event using Cox proportional hazards models analysis (HR = 0.62; 95% CI = 0.41-0.94, p = 0.02). We determined the optimum cutoff value of 1.7 a.u for CUC in the population. Furthermore, the CUC value was important in determining the CVD risk stratification derived from data mining analysis.
Reduced HDL functionality, as measured by CUC, appears to predict CVD in population sample from north-eastern Iran.
高密度脂蛋白（HDL）将胆固醇外排的效率有助于胆固醇逆转运（RCT）途径，这是 HDL 的一个提议功能，并取决于 HDL 摄取胆固醇的能力。我们旨在通过马什哈德（Mashhad Stroke and Heart Atherosclerotic Disorders）队列研究中的样本，用新开发的测定法研究胆固醇摄取能力（CUC）。
研究人群包括 153 名由专家心脏病学家诊断的 CVD 患者，随访时间超过 6 年，以及 350 名无 CVD 的受试者。我们使用改良的 CUC 方法评估血清样本中 HDL 的功能。
CUC 测定法具有很高的可重复性，组内和组间变异系数分别为 13.07 和 6.65。与对照组相比，CVD 组的 CUC 测定值明显较低（p=0.01）。尽管两组之间的血清 HDL-C 没有显著差异，且与进展性 CVD 的风险无显著相关性。多变量逻辑回归分析表明，在调整混杂参数后，CUC 与 CVD 风险之间存在显著负相关（OR=0.57，95%CI=0.38-0.87，p=0.009）。使用 Cox 比例风险模型分析，CUC 也与 CVD 事件的风险呈负相关且独立相关（HR=0.62；95%CI=0.41-0.94，p=0.02）。我们确定了该人群中 CUC 的最佳截断值为 1.7 a.u。此外，CUC 值在确定源自数据挖掘分析的 CVD 风险分层中很重要。
在伊朗东北部人群样本中，CUC 测量的 HDL 功能降低似乎可预测 CVD。
