Gadila Shiva Kumar Goud, Rosoklija Gorazd, Dwork Andrew J, Fallon Brian A, Embers Monica E
Division of Immunology, Tulane National Primate Research Center, Tulane University Health Sciences, Covington, LA, United States.
Department of Psychiatry, Columbia University, New York, NY, United States.
Front Neurol. 2021 May 10;12:628045. doi: 10.3389/fneur.2021.628045. eCollection 2021.
The complex etiology of neurodegenerative disease has prompted studies on multiple mechanisms including genetic predisposition, brain biochemistry, immunological responses, and microbial insult. In particular, Lyme disease is often associated with neurocognitive impairment with variable manifestations between patients. We sought to develop methods to reliably detect , the spirochete bacteria responsible for Lyme disease, in autopsy specimens of patients with a history of neurocognitive disease. In this report, we describe the use of multiple molecular detection techniques for this pathogen and its application to a case study of a Lyme disease patient. The patient had a history of Lyme disease, was treated with antibiotics, and years later developed chronic symptoms including dementia. The patient's pathology and clinical case description was consistent with Lewy body dementia. was identified by PCR in several CNS tissues and by immunofluorescent staining in the spinal cord. These studies offer proof of the principle that persistent infection with the Lyme disease spirochete may have lingering consequences on the CNS.
神经退行性疾病复杂的病因促使人们对多种机制进行研究，包括遗传易感性、脑生物化学、免疫反应和微生物侵害。特别是，莱姆病常与神经认知障碍相关，患者之间的表现各不相同。我们试图开发可靠检测导致莱姆病的螺旋体细菌的方法，用于有神经认知疾病病史患者的尸检标本。在本报告中，我们描述了针对这种病原体的多种分子检测技术的使用及其在一名莱姆病患者病例研究中的应用。该患者有莱姆病病史，接受过抗生素治疗，数年后出现包括痴呆在内的慢性症状。患者的病理学和临床病例描述与路易体痴呆相符。通过PCR在多个中枢神经系统组织中以及通过脊髓免疫荧光染色鉴定出了（此处原文缺失相关病原体名称）。这些研究证明了这样一个原理，即莱姆病螺旋体的持续感染可能会对中枢神经系统产生长期影响。
