Sorbonne Université, INSERM, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France.
IrsiCaixa AIDS Research Institute-HIVACAT, Hospital Universitari Germans Trias i Pujol, Barcelona, Spain.
Front Immunol. 2021 May 10;12:657162. doi: 10.3389/fimmu.2021.657162. eCollection 2021.
The identification of new biomarkers is essential to predict responsiveness to vaccines. We investigated the whole-blood transcriptome and microbiome prior to immunization, in order to assess their involvement in induction of humoral responses two months later. We based our analyses on stool and skin microbiota, and blood transcriptome prior to immunization, in a randomized clinical study in which participants were vaccinated with the MVA-HIV clade B vaccine (MVA-B). We found that the levels of neutralizing antibody responses were correlated with abundance of in stool and in skin. In addition, genus diversity and bacterial species abundance were also correlated with the expression of genes involved in B cell development prior to immunization and forecast strong responders to MVA-B. To our knowledge, this is the first study integrating host blood gene expression and microbiota that might open an avenue of research in this field and to optimize vaccination strategies and predict responsiveness to vaccines.
鉴定新的生物标志物对于预测疫苗的反应性至关重要。我们在免疫前研究了全血转录组和微生物组，以评估它们在两个月后诱导体液反应中的作用。我们的分析基于免疫前的粪便和皮肤微生物组以及血液转录组，在一项随机临床试验中，参与者接种了 MVA-HIV B 型疫苗（MVA-B）。我们发现中和抗体反应的水平与粪便中的丰度和皮肤中的丰度相关。此外，属多样性和细菌物种丰度也与免疫前参与 B 细胞发育的基因表达相关，并预测对 MVA-B 的强反应者。据我们所知，这是首次整合宿主血液基因表达和微生物组的研究，这可能为该领域的研究开辟新途径，并优化疫苗接种策略和预测疫苗的反应性。
