De Vries P, Uytdehaag F G, Osterhaus A D
Department of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Gen Virol. 1988 Aug;69 ( Pt 8):2071-83. doi: 10.1099/0022-1317-69-8-2071.
The potential of immune-stimulating complexes (iscoms), a novel form of antigenic presentation, for the induction of protective immunity against morbillivirus infection was shown by immunizing dogs with canine distemper virus (CDV) iscoms, which contained the fusion (F) protein and a minor amount of the haemagglutinin of the virus. The immunized dogs developed CDV-neutralizing antibodies but, in contrast to non-immunized dogs, did not develop viraemia or clinical signs of infection upon intranasal challenge with the virulent Snyder Hill strain of CDV. Immunization of dogs with measles virus (MV) iscoms, prepared either from affinity-purified MV F protein or from purified whole virus, resulted in partial protection against challenge with CDV. The data presented clearly show that the iscom form of antigenic presentation may be considered a serious candidate for subunit vaccines against morbillivirus infection.
免疫刺激复合物（iscoms）是一种新型的抗原呈递形式，通过用含有融合（F）蛋白和少量病毒血凝素的犬瘟热病毒（CDV）iscoms免疫犬，显示出其诱导针对麻疹病毒感染的保护性免疫的潜力。免疫的犬产生了CDV中和抗体，但与未免疫的犬相比，在用强毒Snyder Hill株CDV进行鼻内攻击后未出现病毒血症或感染的临床症状。用亲和纯化的麻疹病毒（MV）F蛋白或纯化的全病毒制备的MV iscoms免疫犬，可部分保护其免受CDV攻击。所呈现的数据清楚地表明，抗原呈递的iscom形式可被视为抗麻疹病毒感染亚单位疫苗的有力候选者。
