McCulloch Denise J, Jackson Michael L, Hughes James P, Lester Sandra, Mills Lisa, Freeman Brandi, Rasheed Mohammad Ata Ur, Thornburg Natalie J, Chu Helen Y
School of Medicine, University of Washington, Seattle, Washington, United States of America.
Kaiser Permanente Washington, Seattle, Washington, United States of America.
PLoS One. 2021 May 27;16(5):e0252235. doi: 10.1371/journal.pone.0252235. eCollection 2021.
The first US case of SARS-CoV-2 infection was detected on January 20, 2020. However, some serology studies suggest SARS-CoV-2 may have been present in the United States prior to that, as early as December 2019. The extent of domestic COVID-19 detection prior to 2020 has not been well-characterized.
To estimate the prevalence of SARS-CoV-2 antibody among healthcare users in the greater Seattle, Washington area from October 2019 through early April 2020.
We tested residual samples from 766 Seattle-area adults for SARS-CoV-2 antibodies utilizing an ELISA against prefusion-stabilized Spike (S) protein.
No antibody-positive samples were found between October 2, 2019 and March 13, 2020. Prevalence rose to 1.2% in late March and early April 2020.
The absence of SARS-CoV-2 antibody-positive samples in October 2019 through mid-March, 2020, provides evidence against widespread circulation of COVID-19 among healthcare users in the Seattle area during that time. A small proportion of this metropolitan-area cohort had been infected with SARS-CoV-2 by spring of 2020.
2020年1月20日在美国检测到首例严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染病例。然而，一些血清学研究表明，SARS-CoV-2可能早在2019年12月就在美国出现了。2020年之前美国国内新冠病毒病（COVID-19）的检测程度尚未得到充分描述。
估计2019年10月至2020年4月初华盛顿州大西雅图地区医疗保健使用者中SARS-CoV-2抗体的流行率。
我们使用针对预融合稳定刺突（S）蛋白的酶联免疫吸附测定（ELISA）检测了766名西雅图地区成年人的残留样本中的SARS-CoV-2抗体。
在2019年10月2日至2020年3月13日期间未发现抗体阳性样本。2020年3月下旬和4月初流行率升至1.2%。
2019年10月至2020年3月中旬未发现SARS-CoV-2抗体阳性样本，这为当时西雅图地区医疗保健使用者中COVID-19未广泛传播提供了证据。到2020年春季，该大都市地区队列中的一小部分人已感染SARS-CoV-2。
