Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Life Sci. 2021 Aug 15;279:119641. doi: 10.1016/j.lfs.2021.119641. Epub 2021 May 25.
Apigenin (4',5,7-trihydroxyflavone) is one of the subclasses of flavonoids and has various pharmacological effects. The present work was carried out to study the effect of apigenin on ethylene glycol-induced kidney damage in male Wistar rats.
We evaluated the effects of apigenin orally administrated in normal and urolithiatic rats. Animals were assigned to nine groups in random: normal control; apigenin alone (0.005, 0.01, and 0.02 g/kg bw); urolithiatic control (0.75% ethylene glycol and 1.0% ammonium chloride in drinking water); apigenin (0.005, 0.01, and 0.02 g/kg bw) plus ethylene glycol and ammonium chloride; and cystone (0.75 g/kg bw) plus ethylene glycol and ammonium chloride. At the end of 28th day of treatment, animals were sacrificed for biochemical and histopathological assays.
Our results indicated that the apigenin treatment decreased the formation of urinary stones in urolithiatic rats. Also, apigenin reduced the generation of malondialdehyde and enhanced antioxidant enzymes activities in the kidney homogenate of rats. It also caused a significant decrease in the calcium oxalate crystals numbers in urinary sample of rats with ethylene glycol-induced hyperoxaluria. These findings were supported by histopathological examinations.
Based on the results obtained, apigenin attenuate ethylene glycol-related kidney damage in male Wistar rats. Although the underlying mechanism of apigenin effect has not been determined, reduction of urinary levels of stone-producing constituents, antioxidant activities, and inhibition of TGF-β signaling may be involved.
芹菜素（4'，5，7-三羟基黄酮）是黄酮类化合物的一个子类，具有多种药理作用。本研究旨在研究芹菜素对雄性 Wistar 大鼠乙二醇诱导的肾脏损伤的影响。
我们评估了芹菜素口服给予正常和结石形成大鼠的效果。动物随机分为九组：正常对照组；芹菜素单独组（0.005、0.01 和 0.02 g/kg bw）；结石形成对照组（0.75%乙二醇和 1.0%氯化铵饮用水）；芹菜素（0.005、0.01 和 0.02 g/kg bw）加乙二醇和氯化铵；和 cystone（0.75 g/kg bw）加乙二醇和氯化铵。在治疗的第 28 天结束时，处死动物进行生化和组织病理学检测。
我们的结果表明，芹菜素治疗可减少结石形成大鼠的尿结石形成。此外，芹菜素降低了肾脏匀浆中丙二醛的生成，并增强了抗氧化酶的活性。它还导致乙二醇诱导的高草酸尿大鼠尿样中草酸钙晶体数量显著减少。这些发现得到了组织病理学检查的支持。
基于所获得的结果，芹菜素可减轻雄性 Wistar 大鼠乙二醇相关的肾脏损伤。虽然芹菜素作用的潜在机制尚未确定，但减少结石形成成分、抗氧化活性和抑制 TGF-β 信号通路可能参与其中。
