Autonomous Region, Institute of Applied Chemistry, College of Chemistry, Xinjiang University, Urumqi, 830046, Xinjiang, China.
College of Physics and Technology, Xinjiang University, Urumqi, 830046, China.
Biosens Bioelectron. 2021 Oct 1;189:113315. doi: 10.1016/j.bios.2021.113315. Epub 2021 May 18.
As a rapid and non-destructive biological serum detection method, SERS technology was widely used in the screening and medical diagnosis of various diseases by combining the analysis of serum SERS spectrum and multivariate statistical algorithm. Because of the high complexity of serum components and the variability of SERS spectra, which often resulted in the phenomenon that the SERS spectrum of the same biological serum was significantly different due to the different test conditions. In this experiment, through the dilution treatment of the serum and the systematic test of the serum of all concentration gradients with lasers of wavelength of 785, 633 and 532 nm, the most suitable conditions for detecting the serum were investigated. The experimental results showed that only when the serum is diluted to low concentration (10 ppm), the SERS spectrum with high reproducibility and stability could be obtained, furthermore, the low concentration serum had weak tolerance to laser, and 532 nm laser was not suitable for serum detection. In this paper, a set of test scheme for obtaining highly stable serum SERS spectra was established by using high-performance gold nanoparticles (Au NPs) as the active substrate of SERS. Through comparative analysis of SERS spectrum of serum of normal people and cervical cancer, the reliability of the established low-concentration serum test program was verified, as well as its great potential advantages in disease screening and diagnosis.
作为一种快速且无损的生物血清检测方法，SERS 技术通过结合血清 SERS 光谱分析和多元统计算法，被广泛应用于各种疾病的筛选和医学诊断中。由于血清成分的高度复杂性和 SERS 光谱的可变性，经常会出现相同生物血清的 SERS 光谱因测试条件的不同而有显著差异的现象。在本实验中，通过对血清进行稀释处理，并对波长为 785nm、633nm 和 532nm 的激光的所有浓度梯度的血清进行系统测试，研究了最适合检测血清的条件。实验结果表明，只有当血清被稀释到低浓度（10ppm）时，才能获得重现性和稳定性都较高的 SERS 光谱，此外，低浓度血清对激光的容忍度较弱，532nm 激光不适合用于血清检测。本文通过使用高性能金纳米粒子（Au NPs）作为 SERS 的活性基底，建立了一套获得高稳定性血清 SERS 光谱的测试方案。通过对正常人血清和宫颈癌血清的 SERS 光谱进行对比分析，验证了所建立的低浓度血清测试方案的可靠性，以及其在疾病筛查和诊断方面的巨大潜在优势。
