Laboratory of Biological Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Department of Neurobiology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Despota Stefana 142, 11060 Belgrade, Serbia.
Medicina (Kaunas). 2021 May 4;57(5):446. doi: 10.3390/medicina57050446.
: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. : Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. : The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. : Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method's applicability.
: 癌症患者的化疗优化至关重要。由于全球癌症发病率的不断增加，及时且高效的治疗方法不断涌现。在这里，我们提出了一种纳米运动分析方法，该方法可以发展成为一种筛选工具，能够在数小时内为特定患者确定最佳的化疗方案。: 我们使用原子力显微镜 (AFM) 和无尖端的悬臂梁分析了三种不同的人类癌细胞系及其多药耐药 (MDR) 对应物，以附着细胞并监测其纳米运动。: 用阿霉素 (DOX) 处理的细胞因对这种化疗药物的敏感性而产生不同的反应。用 DOX 处理后不到 2 小时，敏感细胞的死亡就会导致信号方差下降，而 MDR 细胞继续运动，甚至信号方差增加。: 纳米运动感应可以发展成为一种筛选工具，能够为每个癌症患者简单、廉价和快速地测试不同的化疗药物。对患者来源的肿瘤细胞的进一步研究应证实该方法的适用性。
