Du Guilin, Zhang Guilong, Shi Jiping, Zhang Jingxian, Ma Zhiguo, Liu Xiangcen, Yuan Chenyang, Li Xiang, Zhang Baoguo
Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai 201210, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Microorganisms. 2021 May 20;9(5):1099. doi: 10.3390/microorganisms9051099.
Ensiling has been widely applied to cope with agricultural solid waste to achieve organic waste valorization and relieve environmental pressure and feedstuff shortage. In this study, co-ensiling of cabbage leaf byproduct and rice straw was performed with inoculation of (LP) to investigate the effects of inoculation on ensiling performance and microflora profiles. Compared to the control, LP inoculation preserved more dry matter (DM) content (283.4 versus 270.9 g·kg fresh matter (FM) on day 30), increased lactic acid (LA) content (52.1 versus 35.8 g·kg dry matter on day 15), decreased pH (3.55 versus 3.79 on day 15), and caused accumulation of acetic acid (AA), butyric acid (BA), and ammonia. The investigation showed that LP inoculation modified microflora composition, especially resisting potential pathogens and enriching more lactic acid bacteria (LAB) ( < 0.05). Moreover, and were identified as the keystone taxa that influenced physicochemical properties and interactions in microflora. They were also the main functional species that directly restrained undesirable microorganisms ( < 0.05), rather than indirectly working via metabolite inhibition and substrate competition ( > 0.05). The results of this present study improve the understanding of the underlying effect of LP inoculation on improving silage quality and facilitate the bio-transformation of cabbage byproduct and rice straw as animal feed.
青贮已被广泛应用于处理农业固体废物，以实现有机废物的增值利用，缓解环境压力和饲料短缺问题。在本研究中，对甘蓝叶副产品和稻草进行混合青贮，并接种植物乳杆菌（LP），以研究接种对青贮性能和微生物群落特征的影响。与对照组相比，接种LP保留了更多的干物质（DM）含量（第30天为283.4克·千克鲜物质（FM），而对照组为270.9克·千克FM），增加了乳酸（LA）含量（第15天为52.1克·千克干物质，而对照组为35.8克·千克干物质），降低了pH值（第15天为3.55，而对照组为3.79），并导致乙酸（AA）、丁酸（BA）和氨的积累。调查表明，接种LP改变了微生物群落组成，特别是抑制了潜在病原体并富集了更多的乳酸菌（LAB）（P<0.05）。此外，植物乳杆菌和嗜酸乳杆菌被确定为影响微生物群落理化性质和相互作用的关键分类群。它们也是直接抑制不良微生物的主要功能物种（P<0.05），而不是通过代谢物抑制和底物竞争间接发挥作用（P>0.05）。本研究结果增进了对LP接种改善青贮质量潜在作用的理解，并促进了甘蓝副产品和稻草作为动物饲料的生物转化。
