González-Pedroza María G, Argueta-Figueroa Liliana, García-Contreras René, Jiménez-Martínez Yaiza, Martínez-Martínez Eduardo, Navarro-Marchal Saúl A, Marchal Juan A, Morales-Luckie Raúl A, Boulaiz Houria
Department of Biotechnology, Faculty of Sciences, Autonomous University of the State of Mexico (UAEMex), Toluca 50200, Mexico.
Conacyt Chairs, Faculty of Dentistry, Benito Juárez Autonomous University of Oaxaca, Oaxaca 68120, Mexico.
Nanomaterials (Basel). 2021 May 12;11(5):1273. doi: 10.3390/nano11051273.
Cancer is one of the most prevalent diseases in the world and requires new therapies for its treatment. In this context, the biosynthesis of silver nanoparticles (AgNPs) has been developed to treat different types of tumors. The plant is known for having anticancer activity. Its main compounds present in the leaves, stems and skin, allowing for its use as reducing agents. In this manuscript, AgNPs with leaf extract (AgNPs-LE) and fruit peel extract (AgNPs-PE) of were biosynthesized obtaining an average nanoparticle diameter sizes smaller than 50 nm, being 19.63 ± 3.7 nm and 16.56 ± 4.1 nm, and with a surface plasmonic resonance (SPR) at 447 and 448 nm, respectively. The lactone functional group present in the LE and PE extracts was identified by the FTIR technique. The behavior and antiproliferation activity of AgNPs-LE and AgNPs-PE were evaluated in breast, colon and melanoma cancer cell lines. Our results showed that fruit peel, which is a waste product, has an antitumor effect more potent than leaf extract. This difference is maintained with AgNPs where the destruction of cancer cells was, for the first time, achieved using concentrations that do not exceed 3 μg/mL with a better therapeutic index in the different tumor strains. In conclusion, we present a low-cost one-step experimental setup to generate AgNPs-PE whose in-vitro biocompatibility and powerful therapeutic effect make it a very attractive tool worth exploiting.
癌症是世界上最普遍的疾病之一，需要新的治疗方法。在此背景下，银纳米颗粒（AgNPs）的生物合成已被开发用于治疗不同类型的肿瘤。这种植物以具有抗癌活性而闻名。其主要化合物存在于叶子、茎和果皮中，可作为还原剂。在本论文中，利用该植物的叶提取物（AgNPs-LE）和果皮提取物（AgNPs-PE）生物合成了AgNPs，得到的纳米颗粒平均直径小于50 nm，分别为19.63±3.7 nm和16.56±4.1 nm，表面等离子体共振（SPR）分别在447和448 nm处。通过傅里叶变换红外光谱（FTIR）技术鉴定了LE和PE提取物中存在的内酯官能团。对AgNPs-LE和AgNPs-PE在乳腺癌、结肠癌和黑色素瘤细胞系中的行为和抗增殖活性进行了评估。我们的结果表明，作为废弃物的果皮具有比叶提取物更强的抗肿瘤作用。在AgNPs中这种差异依然存在，首次使用不超过3μg/mL的浓度实现了对癌细胞的破坏，在不同肿瘤菌株中具有更好的治疗指数。总之，我们提出了一种低成本的一步法实验装置来制备AgNPs-PE，其体外生物相容性和强大的治疗效果使其成为一个非常有吸引力且值得开发的工具。
