MnZnDyFeO（x ≤ 0.1）纳米粒子的抗菌和抗癌活性。

Anti-microbial and anti-cancer activities of MnZnDyFeO (x ≤ 0.1) nanoparticles.

机构信息

Department of Chemistry, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Department of Epidemic Diseases Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

出版信息

Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):493-499. doi: 10.1080/21691401.2021.1938592.

DOI:10.1080/21691401.2021.1938592

PMID:34159846

Abstract

Combining two or more nanoparticles is a promising approach. Previously we have reported synthesis of nanoparticles Dysprosium (Dy) substituted with manganese (Mn) zinc (Zn) by using ultrasonication method. The five different nanoparticles (NPs) MnZnDyFeO ( ≤ 0.1) have been structurally and morphologically characterized but there is no report on the biological application of these NPs. In the present study, we have examined the anti-cancer, anti-bacterial, and anti-fungal activities of MnZnDyFeO ( ≤ 0.1) NPs. Human colorectal carcinoma cells (HCT-116) were tested with different concentrations of NPs by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. In addition, the impact of NPs was also examined on normal cells such as human embryonic kidney cells, HEK-293. After 48 h of treatment, MnZnDyFeO NPs ( = 0.02, 0.04 and 0.06) showed no inhibitory action on cancer cell's growth and proliferation, whereas MnZnDyFeO NPs ( = 0.08 and 0.1) showed profound inhibitory action on cancer cell's growth and proliferation. However, the treatment of MnZnDyFeO NPs on the normal cells (HEK-293) did not show cytotoxic or inhibitory action on HEK-293 cells. The treatment of MnZnDyFeO NPs ( ≤ 0.1) also inhibited both the bacteria ( ATCC35218 and ) with lowest MIC and MBC values of 4 and 8 mg/mL and fungus () with MIC and MFC values of 4 and 8 mg/mL on treatment with = 0.08 and 0. 1.

摘要

将两种或多种纳米粒子结合在一起是一种很有前途的方法。我们之前曾报道过使用超声法合成镝（Dy）取代锰（Mn）锌（Zn）的纳米粒子。已经对五种不同的纳米粒子（NPs）MnZnDyFeO（≤0.1）进行了结构和形态学表征，但尚未有关于这些 NPs 的生物应用的报道。在本研究中，我们研究了 MnZnDyFeO（≤0.1）NPs 的抗癌、抗菌和抗真菌活性。用人结肠癌细胞（HCT-116）通过 MTT[3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐]测定法用不同浓度的 NPs 进行测试。此外，还研究了 NPs 对正常细胞（如人胚肾细胞，HEK-293）的影响。经 48 h 处理后，MnZnDyFeO NPs（=0.02、0.04 和 0.06）对癌细胞的生长和增殖没有抑制作用，而 MnZnDyFeO NPs（=0.08 和 0.1）对癌细胞的生长和增殖有明显的抑制作用。然而，MnZnDyFeO NPs 对正常细胞（HEK-293）的处理并未显示出对 HEK-293 细胞的细胞毒性或抑制作用。MnZnDyFeO NPs（≤0.1）的处理还抑制了细菌（ATCC35218 和）和真菌（），最低 MIC 和 MBC 值分别为 4 和 8 mg/mL，MIC 和 MFC 值分别为 4 和 8 mg/mL，当处理浓度为=0.08 和 0.1 时。