De Matteis Valeria, Rojas Makarena, Cascione Mariafrancesca, Mazzotta Stefano, Di Sansebastiano Gian Pietro, Rinaldi Rosaria
Department of Mathematics and Physics "Ennio De Giorgi", University of Salento, Via Arnesano, 73100 Lecce (LE), Italy.
Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, 73100 Lecce (LE), Italy.
Molecules. 2021 May 13;26(10):2885. doi: 10.3390/molecules26102885.
Noble metals nanoparticles (NPs) and metal oxide NPs are widely used in different fields of application and commercial products, exposing living organisms to their potential adverse effects. Recent evidences suggest their presence in the aquifers water and consequently in drinking water. In this work, we have carefully synthesized four types of NPs, namely, silver and gold NPs (Ag NPs and Au NPs) and silica and titanium dioxide NPs (SiO NPs and TiO NPs) having a similar size and negatively charged surfaces. The synthesis of Ag NPs and Au NPs was carried out by colloidal route using silver nitrate (AgNO) and tetrachloroauric (III) acid (HAuCl) while SiO NPs and TiO NPs were achieved by ternary microemulsion and sol-gel routes, respectively. Once the characterization of NPs was carried out in order to assess their physico-chemical properties, their impact on living cells was studied. We used the human colorectal adenocarcinoma cells (Caco-2), known as the best representative intestinal epithelial barrier model to understand the effects triggered by NPs through ingestion. Then, we moved to explore how water contamination caused by NPs can be lowered by the ability of three species of aquatic moss, namely, , , and to absorb them. The experiments were conducted using two concentrations of NPs (100 μM and 500 Μm as metal content) and two time points (24 h and 48 h), showing a capture rate dependent on the moss species and NPs type. Then, the selected moss species, able to actively capture NPs, appear as a powerful tool capable to purify water from nanostructured materials, and then, to reduce the toxicity associated to the ingestion of contaminated drinking water.
贵金属纳米颗粒（NPs）和金属氧化物纳米颗粒广泛应用于不同的应用领域和商业产品中，使生物体暴露于其潜在的不利影响之下。最近的证据表明它们存在于含水层水中，并因此存在于饮用水中。在这项工作中，我们精心合成了四种类型的纳米颗粒，即银和金纳米颗粒（Ag NPs和Au NPs）以及二氧化硅和二氧化钛纳米颗粒（SiO NPs和TiO NPs），它们具有相似的尺寸和带负电荷的表面。Ag NPs和Au NPs的合成通过胶体途径使用硝酸银（AgNO）和氯金酸（III）（HAuCl）进行，而SiO NPs和TiO NPs分别通过三元微乳液和溶胶 - 凝胶途径实现。一旦对纳米颗粒进行了表征以评估其物理化学性质，就研究了它们对活细胞的影响。我们使用了人结肠腺癌细胞（Caco - 2），它是已知的最好的肠道上皮屏障模型，以了解纳米颗粒通过摄入引发的影响。然后，我们着手探索三种水生苔藓，即 、 和 吸收纳米颗粒的能力如何降低纳米颗粒引起的水污染。实验使用两种纳米颗粒浓度（作为金属含量为100μM和500μM）和两个时间点（24小时和48小时）进行，结果表明捕获率取决于苔藓种类和纳米颗粒类型。然后，能够主动捕获纳米颗粒的选定苔藓种类似乎是一种强大的工具，能够从纳米结构材料中净化水，进而降低与摄入受污染饮用水相关的毒性。
