Chanayat Yuttana, Akatvipat Areerath, Bender Jeff B, Punyapornwithaya Veerasak, Meeyam Tongkorn, Anukool Usanee, Pichpol Duangporn
Master's Degree Program in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Department of Companion Animals and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Vet Sci. 2021 May 13;8(5):85. doi: 10.3390/vetsci8050085.
This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome (SCC) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. detection and staphylococcal cassette chromosome (SCC) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The and class-C2 , which were SCC type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCC type V was found in , group, , and . Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCC MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.
本研究对从犬浅表脓皮病感染中分离出的临床耐甲氧西林葡萄球菌（MRS）进行了特征分析。我们的目的是确定临床病例中MRS分离株的葡萄球菌盒式染色体（SCC）类型和抗菌药敏性。收集皮肤拭子并进行培养。通过生化试验、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定和表征菌种，并采用纸片扩散法进行抗菌药敏试验。通过聚合酶链反应（PCR）进行检测和葡萄球菌盒式染色体（SCC）分型。在65份临床样本中，鉴定出56份（86.2%）葡萄球菌感染。56株分离株中有12株（21%）为MRS感染。所有MRS分离株均对多种药物耐药。在12株MRS分离株中，SCC V型的 mecA 和 C2 类最常见（66.7%）。在金黄色葡萄球菌、中间葡萄球菌组、溶血葡萄球菌、施氏葡萄球菌中发现了主要的SCC V型。随着与浅表脓皮病相关的犬中高耐药MRS的出现，治疗失败成为一个问题。此前在兽医和犬主中曾报道过V型SCC MRS的检测，但在泰国北部尚未见报道。这些感染提醒我们要改进感染预防和控制措施, 包括减少环境污染以及MRS的潜在人畜共患病暴露。此外，需要提高兽医医院工作人员、客户和患者对小动物医院这些风险的教育意识。
