González-Domínguez María S, Carvajal Hernán D, Calle-Echeverri David A, Chinchilla-Cárdenas Danny
INCA-CES Research Group, Veterinary Teaching Hospital, Faculty of Veterinary Medicine and Zootechny, CES University, Medellín, Colombia.
Instituto Colombiano de Medicina Tropical (ICMT), CES University, Medellín, Colombia.
Front Vet Sci. 2020 Jul 23;7:376. doi: 10.3389/fvets.2020.00376. eCollection 2020.
Canine superficial pyoderma (CSP) is a bacterial infection secondary to several skin diseases of the dog. , which is a commensal bacterium of the dog's skin, is the leading agent found in dogs affected by CSP, which can progress to deep pyoderma. It is also of clinical significance because . strains carry antimicrobial resistance genes, mainly the gene. In this descriptive longitudinal study, molecular characterization of bacterial isolates from dogs affected by CSP was performed in addition to phenotyping, antimicrobial profiling, and assessment of resistance carriage status. Fifty dogs (24 females and 26 males) attending the CES University Veterinary Teaching Hospital were included in the study. CSP was confirmed according to clinical signs and cytological examination. Swabs were taken from active skin lesions for bacterial culture, and phenotyping and antimicrobial resistance profiles were assessed using API-Staph phenotyping and the Kirby-Bauer method, respectively. We also performed molecular detection and characterization of the and encoding gene of coagulase-positive Staphylococci. The gene frequency was established by qPCR amplification of a 131bp gene fragment. Data were evaluated by descriptive statistics. Erythema, peeling, pruritus, and alopecia were the predominant symptoms (72, 56, and 46%, respectively). We isolated bacteria compatible with species from all samples tested. API phenotyping showed 83.1 to 97.8% compatibility with . PCR-genotyping resulted in 15, 3, and 1 isolates positive for , and , respectively. Isolated strains showed high susceptibility to Imipenem, Ampicillin/Sulbactam, and Rifampicin (100, 94, and 92%, respectively). The highest resistance was against Vancomycin and Trimethoprim/Sulfamethoxazole (98 and 74%, respectively). , and isolates were cloned and shared 96% sequence homology. Finally, we found 62% carriage status of the gene in isolates of CSP patients, although only 36% of the isolates were methicillin-resistant. Identification of three species causing CSP, high-level resistance against conventional antimicrobials, and carriage of the gene highlight the importance of performing molecular characterization of bacteria causing dermatological conditions in dogs.
犬浅表脓皮病（CSP）是继发于犬的几种皮肤病的细菌感染。 是犬皮肤的共生菌，是受CSP影响的犬中发现的主要病原体，可发展为深部脓皮病。它也具有临床意义，因为 菌株携带抗菌抗性基因，主要是 基因。在这项描述性纵向研究中，除了表型分析、抗菌谱分析和抗性携带状态评估外，还对受CSP影响的犬的细菌分离株进行了分子特征分析。该研究纳入了CES大学兽医教学医院的50只犬（24只雌性和26只雄性）。根据临床症状和细胞学检查确诊为CSP。从活跃的皮肤病变处采集拭子进行细菌培养，并分别使用API - Staph表型分析和Kirby - Bauer方法评估表型和抗菌抗性谱。我们还对凝固酶阳性葡萄球菌的 和 编码基因进行了分子检测和特征分析。 通过对131bp基因片段的qPCR扩增确定 基因频率。数据通过描述性统计进行评估。红斑、脱皮、瘙痒和脱毛是主要症状（分别为72%、56%和46%）。我们从所有测试样本中分离出了与 种相容的细菌。API表型分析显示与 的相容性为83.1%至97.8%。PCR基因分型结果显示， 、 和 分别有15株、3株和1株阳性。分离菌株对亚胺培南、氨苄西林/舒巴坦和利福平表现出高敏感性（分别为100%、94%和92%）。对万古霉素和甲氧苄啶/磺胺甲恶唑的抗性最高（分别为98%和74%）。 、 和 的分离株被克隆，序列同源性为96%。最后，我们发现CSP患者分离株中 基因的携带状态为62%，尽管只有36%的分离株对甲氧西林耐药。鉴定出三种导致CSP的 种、对传统抗菌药物的高水平抗性以及 基因的携带突出了对引起犬皮肤病的细菌进行分子特征分析的重要性。
