Teixeira Susana A, Marques Daniele B D, Costa Thaís C, Oliveira Haniel C, Costa Karine A, Carrara Eula R, Silva Walmir da, Guimarães José D, Neves Mariana M, Ibelli Adriana M G, Cantão Maurício E, Ledur Mônica C, Peixoto Jane O, Guimarães Simone E F
Department of Animal Science, Universidade Federal de Viçosa, Viçosa 36570-000, MG, Brazil.
Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa 36570-000, MG, Brazil.
Animals (Basel). 2021 May 18;11(5):1443. doi: 10.3390/ani11051443.
Since pre- and postnatal development are programmed during early prenatal life, studies addressing the complete transcriptional landscape during organogenesis are needed. Therefore, we aimed to disentangle differentially expressed (DE) genes between fetuses (at 35 days old) and embryos (at 25 days old) through RNA-sequencing analysis using the pig as model. In total, 1705 genes were DE, including the top DE , , , , , and genes, which are associated with developmental transition from embryos to fetuses, such as ossification, skeletal muscle development, extracellular matrix organization, cardiovascular system, erythrocyte differentiation, and neuronal system. In pathway analysis, embryonic development highlighted those mainly related to morphogenic signaling and cell interactions, which are crucial for transcriptional control during the establishment of the main organs in early prenatal development, while pathways related to myogenesis, neuronal development, and cardiac and striated muscle contraction were enriched for fetal development, according to the greater complexity of organs and body structures at this developmental stage. Our findings provide an exploratory and informative transcriptional landscape of pig organogenesis, which might contribute to further studies addressing specific developmental events in pigs and in other mammals.
由于产前和产后发育在产前早期就已编程，因此需要开展针对器官发生过程中完整转录图谱的研究。因此，我们旨在以猪为模型，通过RNA测序分析来厘清胎儿（35日龄）和胚胎（25日龄）之间的差异表达（DE）基因。总共1705个基因存在差异表达，包括排名靠前的差异表达基因，这些基因与从胚胎到胎儿的发育转变相关，如骨化、骨骼肌发育、细胞外基质组织、心血管系统、红细胞分化和神经系统。在通路分析中，胚胎发育突出显示了那些主要与形态发生信号传导和细胞相互作用相关的通路，这些通路对于产前早期主要器官形成过程中的转录控制至关重要，而根据该发育阶段器官和身体结构更复杂的情况，与肌发生、神经元发育以及心脏和横纹肌收缩相关的通路在胎儿发育中得到富集。我们的研究结果提供了猪器官发生的探索性且信息丰富的转录图谱，这可能有助于进一步研究猪和其他哺乳动物中的特定发育事件。
