School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Korea.
Int J Mol Sci. 2021 May 18;22(10):5316. doi: 10.3390/ijms22105316.
In the last two decades, global environmental change has increased abiotic stress on plants and severely affected crops. For example, drought stress is a serious abiotic stress that rapidly and substantially alters the morphological, physiological, and molecular responses of plants. In Arabidopsis, several drought-responsive genes have been identified; however, the underlying molecular mechanism of drought tolerance in plants remains largely unclear. Here, we report that the "domain of unknown function" novel gene () positively regulates drought stress in Arabidopsis. The Arabidopsis loss-of-function mutant atduf569 showed significant sensitivity to drought stress, i.e., severe wilting at the rosette-leaf stage after water was withheld for 3 days. Importantly, the mutant plant did not recover after rewatering, unlike wild-type (WT) plants. In addition, plants showed significantly lower abscisic acid accumulation under optimal and drought-stress conditions, as well as significantly higher electrolyte leakage when compared with WT Col-0 plants. Spectrophotometric analyses also indicated a significantly lower accumulation of polyphenols, flavonoids, carotenoids, and chlorophylls in mutant plants. Overall, our results suggest that novel is a positive regulator of the response to drought in Arabidopsis.
在过去的二十年中，全球环境变化增加了植物的非生物胁迫，并严重影响了作物。例如，干旱胁迫是一种严重的非生物胁迫，它会迅速而显著地改变植物的形态、生理和分子反应。在拟南芥中，已经鉴定出了一些对干旱响应的基因；然而，植物抗旱性的潜在分子机制在很大程度上仍不清楚。在这里，我们报告“未知功能”新型基因 () 正向调控拟南芥的干旱胁迫反应。拟南芥功能丧失突变体 atduf569 对干旱胁迫表现出显著的敏感性，即在断水 3 天后，莲座叶期的植株严重萎蔫。重要的是，与野生型 (WT) 植物不同，突变体植物在重新浇水后无法恢复。此外，与 WT Col-0 植物相比，在最佳和干旱胁迫条件下，突变体植物中的脱落酸积累明显较低，而电解质渗漏明显较高。分光光度分析还表明，突变体植物中多酚、类黄酮、类胡萝卜素和叶绿素的积累明显较低。总的来说，我们的结果表明 novel 是拟南芥响应干旱的正调控因子。
